Community analysis of sulfate-reducing bacteria during methanogenic process in paddy field soil
| Project/Area Number |
26450077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ASAKAWA Susumu 名古屋大学, 大学院生命農学研究科, 教授 (50335014)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 水田土壌 / 硫酸還元菌 / 水素生成 / 群集構造 / DGGE |
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| Outline of Final Research Achievements |
This study aimed to reveal community structure of sulfate-reducing bacteria and their roles in paddy field soil. Community analysis showed that diverse sulfate-reducing bacteria including uncultured and unknown members inhabited paddy field soils and the composition and dynamics were different depending on the fields. Analysis of hydA gene, which encode [FeFe]-hydrogenase known as an H2-producing enzyme, and the transcripts of a sulfate-reducing bacterium isolated from a paddy field soil revealed that the bacterium differently regulated transcription of two hydA paralogs between under sulfate-reducing and H2-producing conditions. In addition, hydA genes related to sulfate-reducing bacteria were transcribed in an anoxic paddy soil. These findings indicated that sulfate-reducing bacteria are involved in the anaerobic decomposition of organic material in paddy field soil as one of the key H2-producers.
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Report
(4 results)
Research Products
(11 results)
