| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To avoid lethal depletion of cellular Zn, plants modulate the molecular mechanism of Zn homeostasis. To explore the genes that participate in Zn homeostasis under Zn-deficient condition, we have performed iTRAQ-based quantitative proteomics using microsomal proteins from the roots of A. thaliana wild-type and bzip19 mutant. Through this analysis, it has revealed that expressions of several Zn transporters and defensin-like proteins were regulated by bZIP19 and bZIP23. This result has supported by selected reaction monitoring analysis. Also, it had showed that bZIP19 and bZIP23 independently regulate their own target genes to adapt to Zn-deficient condition, although it has reported that they have redundant functions on the regulation of Zn transporters in a previous report. Furthermore, it has suggested that the defensin-like proteins contribute to Zn homeostasis.
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