| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the key enzyme for atmospheric CO2 fixation. The Arabidopsis bsd2 mutant showed a significant reduction in photosynthetic capacity and was unable to grow autotrophically. BSD2 directly interacts with Rubisco, and recombinant BSD2 could reduce the oxidized form of Rubisco in vitro via disulfide reductase activity. A role for BSD2 in maintaining Rubisco activity was further supported in Arabidopsis BSD2-overexpression lines where Rubisco activation status and photosynthetic activity were higher than wild type. Our results demonstrate that BSD2 is key in the mechanisms that regulates the reactivation of oxidized Rubisco, thereby maintaining functional photosynthesis in plants.
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