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Construction of a microbial flora dynamics model in nitrogen-fixing bacterial symbiotic system and conversion of atmospheric nitrogen

Research Project

Project/Area Number 26450085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionUtsunomiya University

Principal Investigator

Maeda Isamu  宇都宮大学, 農学部, 准教授 (10252701)

Co-Investigator(Renkei-kenkyūsha) WATANABE Masanori  山形大学, 農学部, 准教授 (20320020)
Research Collaborator KUGENUMA Takumi  
ARASHIDA Haruka  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords窒素固定 / 光合成細菌 / 枯草菌 / 共培養 / ニトロゲナーゼ / 共生系 / 枯草菌―紅色非硫黄細菌共生系 / 枯草菌―光合成細菌共生系 / 細菌共生系
Outline of Final Research Achievements

Ammonia, a raw material for nitrogen fertilizer, is synthesized from nitrogen and hydrogen by chemical synthesis. The biological nitrogen fixing reaction, which synthesizes ammonia from nitrogen in the air under milder conditions, is catalyzed by diazotrophic bacteria, and the reaction is inhibited by oxygen. This study demonstrates that by introducing co-culture with Bacillus subtilis, which can grow with oxygen consumption, photosynthetic bacteria, which belong to a group of diazotrophic bacteria, actively fixed nitrogen in the presence of air and grew diazotrophically after the growth of B. subtilis. From this result, development of microbial fertilizer as a nitrogen source is expected.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] 微生物共培養による窒素固定能の発現: 微生物共生体における窒素からアンモニアへの変換2017

    • Author(s)
      前田 勇
    • Journal Title

      化学と生物

      Volume: 55 Pages: 113-118

    • NAID

      130006315945

    • Related Report
      2016 Research-status Report
  • [Journal Article] Light-enhanced bioaccumulation of molybdenum by nitrogen-deprived recombinant anoxygenic photosynthetic bacterium Rhodopseudomonas palustris.2016

    • Author(s)
      Taki Naito, Sachuronggui, Masayoshi Ueki, and Isamu Maeda
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 2 Pages: 407-413

    • DOI

      10.1080/09168451.2015.1086260

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 空気封入下の光合成細菌-枯草菌共培養におけるニトロゲナーゼ活性発現2017

    • Author(s)
      前田 勇, 嵐田 遥, 渡辺 昌規
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光合成細菌と枯草菌の共培養による酸素ガス共存下の窒素固定2016

    • Author(s)
      前田 勇,久下沼 匠
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 大気中窒素ガスをN源とした枯草菌―紅色非硫黄細菌共生系の増殖評価2016

    • Author(s)
      前田 勇,久下沼 匠
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 大気中窒素分子をN源とした紅色非硫黄細菌の増殖能の評価2014

    • Author(s)
      前田 勇,久下沼 匠
    • Organizer
      第66回(2014年)日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Remarks] 光合成細菌と枯草菌の共培養による窒素固定

    • URL

      http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/chemj/jmicrobio/microb-eng1.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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