| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Arsenic (As) is released from anaerobic sediments into groundwater as arsenite, and dissimilatory arsenate-reducing bacteria (DARB) may play important roles in As release. Geobacter sp. OR-1, one of DARB, has a variety of As-metabolizing genes such as dissimilatory arsenate reductase genes (arrAB) and genes for As resistance (arsACDR) in its genome. The aim of this study was to understand expression dynamics of these genes or proteins in strain OR-1. Transcriptional analysis revealed that most of As-metabolizing genes were induced by As, and that the expression of arrA and arsC was 52 and 260 times higher in the presence of As, respectively. Comprehensive proteomic analysis showed that proteins involved in oxidative stress response, protein folding, molecular chaperones, and sulfur metabolism were also expressed cooperatively in the presence of As. Our results shed light on how microbes cope with this toxic metalloid, and play an important role in the transfer of As in nature.
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