Environmental sulfur sensing by sulfite signaling, and control mechanism of sulfur assimilation
| Project/Area Number |
26450091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba (2016)
Nara Institute of Science and Technology (2014-2015) |
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Principal Investigator |
OHTSU Iwao 筑波大学, 国際産学連携本部, 准教授 (60395655)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 硫黄同化経路 / チオ硫酸イオン / 亜硫酸イオン / 硫化水素 / 大腸菌 / システイン / 活性硫黄種 / 硫黄代謝 / メタボローム / シグナル分子 / 硫黄同化（代謝） / システイン（代謝） / アミノ酸発酵 / レポーターアッセイ / 遺伝子発現 / チオ硫酸 / 硫酸 |
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| Outline of Final Research Achievements |
We report the identification of a novel regulation system of sulfite (SO3) or hydrogen sulfide (H2S) production in E. coli. We have measured the capacity of H2S generation in the medium containing glucose, and showed that glucose limitation induces abundant production of H2S from E. coli cells. No requirement of cysteine degradation for this inducible H2S generation because effect of deletion of genes involved cysteine degradation is negligible. Experiments using minimal synthetic medium containing inorganic sulfur compounds as a sulfur source revealed that the H2S is produced by assimilation of thiosulfate, but not by sulfate. We also found that Crp is required for the H2S production to response in glucose limitation. These results indicates that, in E. coli, carbon and sulfur metabolism are likely to be coordinately controlled by sensing starvation for nutrients.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] A finding of thiosulfate pathway for synthesis of organic sulfur compounds in Saccharomyces cerevisiae and an improvement of ethanol production.2015
Author(s)
Eri Funahashi, Kyohei Saiki, Kurara Honda, Yuki Sugiura, Yusuke Kawano, Iwao Ohtsu, Daisuke Watanabe, Yukari Wakabayashi, Tetsuya Abe, Tsuyoshi Nakanishi, Makoto Suematsu, and Hiroshi Takagi
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Journal Title
J. Biosci. Bioeng.
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Involvement of the yciW gene in L-cysteine and l-methionine metabolism in Escherichia coli.2015
Author(s)
Yusuke Kawano, Iwao Ohtsu, Ai Tamakoshi, Maeka Shiroyama, Ai Tsuruoka, Kyouhei Saiki, Kazuhiro Takumi, Gen Nonaka, Tsuyoshi Nakanishi, Takako Hishiki, Makoto Suematsu, and Takagi Hiroshi.
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Journal Title
J. Biosci. Bioeng.
Volume: １１９
Pages: 310-313
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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