| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We report the identification of a novel regulation system of sulfite (SO3) or hydrogen sulfide (H2S) production in E. coli. We have measured the capacity of H2S generation in the medium containing glucose, and showed that glucose limitation induces abundant production of H2S from E. coli cells. No requirement of cysteine degradation for this inducible H2S generation because effect of deletion of genes involved cysteine degradation is negligible. Experiments using minimal synthetic medium containing inorganic sulfur compounds as a sulfur source revealed that the H2S is produced by assimilation of thiosulfate, but not by sulfate. We also found that Crp is required for the H2S production to response in glucose limitation. These results indicates that, in E. coli, carbon and sulfur metabolism are likely to be coordinately controlled by sensing starvation for nutrients.
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