Elucidation of molecular mechanisms on the stress response in oleaginous microorganism thraustochytrid and its application to value-added lipid production
| Project/Area Number |
26450092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
Aki Tsunehiro 広島大学, 先端物質科学研究科, 教授 (80284165)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 脂質生合成 / ラビリンチュラ / オーランチオキトリウム / カロテノイド / 遺伝子発現調節 / 酵素機能 / 脂質 / 高度不飽和脂肪酸 / 炭化水素 |
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| Outline of Final Research Achievements |
This research aims to elucidate the molecular mechanisms underlying the stress response nature of carotenoid production which has been suggested in transcriptome of marine thraustochytrid, Aurantiochytrium. Analysis on the functional domain disruptants of β-carotene synthase CrtIBY did not suggest any cooperative action between three domains. A luciferase reporter system was constructed to investigate the regulation mechanisms of gene expression of CrtIBY. This system reproduced the expression pattern of CrtIBY gene, being enhanced at the stationary and starvation phases of cultivation.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Dysgonomonas alginatilytica sp. nov., a novel alginate-degrading bacterium isolated from microbial consortium2015
Author(s)
A. Kita, T. Miura, S. Kawata, K. Yamaguchi, Y. Okamura, T. Aki, Y. Matsumura, T. Tajima, J. Kato, Y. Nakashimada
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Journal Title
Int. J. Sys. Evol. Microbiol.
Volume: 65
Issue: Pt_10
Pages: 3570-3575
DOI
Related Report
Peer Reviewed
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[Presentation] Bioconversion of marine biomass for production of valuable lipids2015
Author(s)
K.H.V. Arafiles, R. Sato, A. Nagano, Y. Eramoto, A. Oda, R. Higashi, H. Iwasaka, K. Watanabe, Y. Okamura, T. Aki
Organizer
11th International Symposium on Biocatalysis and Agricultural Biotechnology
Place of Presentation
Banff, Canada
Year and Date
2015-09-14
Related Report
Int'l Joint Research / Invited
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