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Elucidation of the growth stimulating mechanism of previously uncultured microorganisms

Research Project

Project/Area Number 26450093
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

MURAKAMI CHIHO  広島大学, 工学研究科, 日本学術振興会特別研究員(RPD) (50649077)

Co-Investigator(Kenkyū-buntansha) 青井 議輝  広島大学, 先端物質科学研究科, 准教授 (40386636)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords難培養性微生物 / 亜硝酸酸化細菌 / 増殖促進因子 / 共生
Outline of Final Research Achievements

Previously uncultured microorganisms Nitrospira which are also nitrite oxidizing microorganisms resist the cultivation of agar plates. Nitrospira are co-existing with heterotrophs in the enrichment culture. Growth of those heterotrophs are completely dependent on the organic compounds excreted from autotrophs as metabolic by-products. However, it is unclear whether the symbiotic relationships are commensal or mutualistic.
Then we screened 16 isolates of heterotrophs, co-cultured with and tested growth promoting activity on Nitrospira. The growth of Nitrospira was clearly stimulated by co-cultivation with 4 isolates, suggesting to have a benefit to be co-existing with each other. We next tested those isolates to identify whether the growth promoting on Nitrospira are derived from, 1) removal of inhibiting factor for Nitrospira or, 2) supply of growth promoting factor. The result suggested that heterotrophs provide unknown growth promoting factor to Nitrospira.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015 2014 Other

All Presentation (8 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Presentation] 亜硝酸酸化細菌の休眠と覚醒 -未知増殖制御メカニズムの解明-2018

    • Author(s)
      寺地裕康 村上千穂 金田一智規 大橋晶良 青井議輝
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 難培養性微生物 Nitrospira の増殖促進・覚醒に関与する因子の解明2017

    • Author(s)
      村上千穂 金田一智規 町田光史 中尾洋一 大橋晶良 青井議輝
    • Organizer
      第2回環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 難培養性微生物 Nitrospira の休眠現象2017

    • Author(s)
      寺地裕康 村上千穂 金田一智規 大橋晶良 青井議輝
    • Organizer
      第2回環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 難培養性微生物 Nitrospira の増殖促進・休眠と覚醒に関与する因子の解明2017

    • Author(s)
      村上千穂 金田一智規 町田光史 中尾洋一 大橋晶良 青井議輝
    • Organizer
      特殊環境微生物セミナー 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 難培養性微生物の増殖促進、休眠・覚醒現象の解明2016

    • Author(s)
      村上千穂 金田一智規 大橋晶良 青井議輝
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      横須賀文化会館
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Growth-promoting, dormancy and awakening of previously uncultured microorganisms Nitrospira2015

    • Author(s)
      村上千穂 金田一智規 大橋晶良 青井議輝
    • Organizer
      2015日本微生物生態学会第30回大会(7th Japan-Taiwan-Korea Symposium on Microbial Ecology)
    • Place of Presentation
      茨城県土浦市亀城プラザ
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] How do microorganisms sleep and wake up?2014

    • Author(s)
      Chiho Murakami Tomonori Kindaichi Akiyoshi Ohashi Yoshiteru Aoi
    • Organizer
      2nd HIROSHIMA INTERNATIONAL SYMPOSIUM ON SUSTANABILITY SCIENCES
    • Place of Presentation
      広島大学
    • Year and Date
      2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 難培養性微生物の増殖活性化に寄与する異種間相互作用2014

    • Author(s)
      村上千穂 金田一智規 大橋晶良 青井議輝
    • Organizer
      環境微生物系学会合同大会
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Remarks] AOI LAB.

    • URL

      http://home.hiroshima-u.ac.jp/sust6aoi/ja/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 広島大学サステナブル・ディベロップメント実践研究センター AOILAB

    • URL

      http://home.hiroshima-u.ac.jp/sust6aoi/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2020-12-14  

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