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Biochemical analysis of oxidative fermentation of novel oxidized sugars having an intramolecular ketone

Research Project

Project/Area Number 26450094
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionYamaguchi University

Principal Investigator

ADACHI OSAO  山口大学, その他部局等, 名誉教授 (20027189)

Co-Investigator(Kenkyū-buntansha) 赤壁 善彦  山口大学, 創成科学研究科, 教授 (20274186)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords酸化発酵 / 酢酸菌 / 分子内ケトンをもつ酸化糖 / 5-ケトプシコース / 4-ケトペント酸 / Ga. liquefaciens / 酸化糖 / ヒドロキシピルビン酸 / 廃グリセリン / 産業廃棄物 / 膜結合型脱水素酵素 / 4-ケトアラボン酸 / 4-ケトリボン酸 / 5-ケトフルクトース / カルボニル酸化糖 / PQQ酵素 / デキストラン合成酵素
Outline of Final Research Achievements

A novel oxidation of pentonates to 4-keto- pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca2+. The enzyme oxidized pentonates to 4-keto-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized fructose to 5-keto- fructose, psicose to 5-keto-psicose, including the other polyols such as, glycerol, ribitol, arabitol, and sorbitol. Thus, pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH). The reaction versatility of quinoprotein GLDH was notified in this study. Oxidative fermentation of hydroxypyruvate was examined and two oxidizing systems have been indicated: System 1: Glycerol - Dihydroxyacetone - Hydroxypyruvic aldehyde - Hydroxypyruvic acid. System 2: Glycerol - Glyceric acid - Hydroxymalonaldehyde - Hydroxymalonic acid

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (5 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] National Univ. La Plata/CINDEFI/National Univ. Misiones(Argentina)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Mem brane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonbates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose.2017

    • Author(s)
      Y. Ano, R.A. Hours, Y. Akakabe, N. Kataoka, T. Yakushi, K. Matsushita, O. Adachi
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 81 Issue: 2 Pages: 411-418

    • DOI

      10.1080/09168451.2016.1254535

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Study of the chlorogenic acid content in yerba mate (Ilex paraguariensis St. Hil.): effect of plant fraction, processing step and harvesting season.2016

    • Author(s)
      AP. Butiuk, MA. Martos, O. Adachi. RA. Hours
    • Journal Title

      J. Appl. Res. Medici. Arom. Plants.

      Volume: 3 Issue: 1 Pages: 27-33

    • DOI

      10.1016/j.jarmap.2015.12.003

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Yerba mate as a novel inducer for fungal chlorogenate hydrolase production.2015

    • Author(s)
      A.P. Butiuk, O. Adachi, H.A. Hours.
    • Journal Title

      Biocatal. Agric. Biotechnol.,

      Volume: 4 Issue: 3 Pages: 327-334

    • DOI

      10.1016/j.bcab.2015.06.002

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Reduction of L-phenylalanine in protein hydrolysates using L-phenylalanine ammonia-lyase from Rhodosporidium toruloides2015

    • Author(s)
      M.T. Castenada, O. Adachi, R.A. Hours.
    • Journal Title

      J. Ind. Microbial Biotechnol.

      Volume: 42 Issue: 10 Pages: 1299-1307

    • DOI

      10.1007/s10295-015-1664-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Acetic acid bacteria: A group of bacteria with versatile biotechnological applications.2015

    • Author(s)
      Saichana N, Matsushita K, Adachi O, Frebort I, Frebortova J.
    • Journal Title

      Biotechnol Adv.

      Volume: in press Issue: 6 Pages: 1260-1271

    • DOI

      10.1016/j.biotechadv.2014.12.001

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 酢酸菌Gluconobacter thailandicusにおけるジヒドロキシアセトン代謝に関する研究:ジヒドロキシアセトン・リン酸化酵素の遺伝学的・生化学的解析2017

    • Author(s)
      薬師寿治、平田花織、松谷峰之介、足立収生、片岡尚也、松下一信
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都女子大学(京都府京都市東山区)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gluconobacter 属酢酸菌キノプロテイン・グリセロール脱水素酵素における新たな基質の発見とその反応メカニズムの提唱2017

    • Author(s)
      寺田優花、尾崎聖士郎、片岡尚也、足立収生、赤壁善彦、薬師寿治、松下一信
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都女子大学(京都府京都市東山区)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酢酸菌における複数の新規なPQQ-酵素の同定2016

    • Author(s)
      足立収生、R.A. Hours、赤壁善彦、阿野嘉孝、品川恵美子、片岡尚也、薬師寿治、松下一信
    • Organizer
      日本農芸化学会H28年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 膜結合型デキストラン合成酵素の発見とその機能解析2015

    • Author(s)
      足立収生、R.A. Hours、赤壁善彦、阿野嘉孝、品川恵美子、片岡尚也、薬師寿治、松下一信
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Optimizacion de la actividad clorogenato hidrolasa de aspergillus niger para la bioconversion del acido clorogenico de la yerba mate2014

    • Author(s)
      A.P. Butiuk, S.A. Midana, M.A. Martos, O. Adachi, R.A. Hours
    • Organizer
      VI Congreso Sudamericano de Yerba Mate/II Simposio Internacional de Yerba Mate y Salud
    • Place of Presentation
      Hotel Radisson, Montevideo, Uruguay
    • Year and Date
      2014-05-09
    • Related Report
      2014 Research-status Report
  • [Book] Acetic Acid Bacteria.2016

    • Author(s)
      O. Adachi, T. Yakushi.
    • Publisher
      John Wiley Publishing.
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] デキストランの製造方法2015

    • Inventor(s)
      足立収生、松下一信、薬師寿治、片岡尚也
    • Industrial Property Rights Holder
      山口大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-037541
    • Filing Date
      2015-02-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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