The role of clostridial T-A system

• Project/Area Number: 26450096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagawa University
• Principal Investigator: Suzuki Motoo 香川大学, 医学部, 助教 (80457340)
• Co-Investigator(Kenkyū-buntansha): 桑原 知巳 香川大学, 医学部, 教授 (60263810) ; 今大路 治之 (中山治之 / 中山 治之) 香川大学, 医学部, 助教 (80294669)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: SFB / 胞子 / 難培養

Outline of Final Research Achievements

Segmented filamentous bacteria (SFBs) are non-culturable spore-forming bacteria, tightly attaching to the intestinal epithelial cells. SFBs play crucial roles in the maturation of gut immune function, especially the induction of Th17 lymphocytes. However, we found the phenomenon that Th17-induction of SFBs was not detected by repeating fecal passages in germ-free mice.
We suspected that Th17-uninducible SFB strain was selected from multiple clones, since we observed many single nucleotide polymorphisms in DNA sample obtained for whole-genome sequencing analysis. To clarify the effect of each SFB strain on Th-17 induction, we attempted to isolate single-clone SFB. We have established the method for purifying spores from feces of SFB-gnotobiotic mice. Furthermore, we have isolated single-clone SFB by inoculating the diluted purified spore into germ-free mice.

Research Products

• [Presentation] マウス腸内難培養菌の胞子精製 (2014)
  • Author(s): 鈴木基生; 今大路治之; 桑原知巳
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 長浜バイオ大学（滋賀県長浜市）
  • Year and Date: 2014-09-17 – 2014-09-19