| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Segmented filamentous bacteria (SFBs) are non-culturable spore-forming bacteria, tightly attaching to the intestinal epithelial cells. SFBs play crucial roles in the maturation of gut immune function, especially the induction of Th17 lymphocytes. However, we found the phenomenon that Th17-induction of SFBs was not detected by repeating fecal passages in germ-free mice.
We suspected that Th17-uninducible SFB strain was selected from multiple clones, since we observed many single nucleotide polymorphisms in DNA sample obtained for whole-genome sequencing analysis. To clarify the effect of each SFB strain on Th-17 induction, we attempted to isolate single-clone SFB. We have established the method for purifying spores from feces of SFB-gnotobiotic mice. Furthermore, we have isolated single-clone SFB by inoculating the diluted purified spore into germ-free mice.
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