Study on the production of rare disaccharides and rare sugars.

• Project/Area Number: 26450097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagawa University
• Principal Investigator: Takata Goro 香川大学, 国際希少糖研究教育機構, 准教授 (50322722)
• Co-Investigator(Kenkyū-buntansha): 森本 兼司 香川大学, 国際希少糖研究教育機構, 准教授 (90363184) ; 上地 敬子 香川大学, 希少糖研究センター, 博士研究員 (70733426)
• Co-Investigator(Renkei-kenkyūsha): Sakuraba Haruhiko 香川大学, 農学部, 准教授 (90205823)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 希少糖 / D-アロース / 希少二糖 / D-グルコシド3-デヒドロゲナーゼ

Outline of Final Research Achievements

The objective of this study is the production of D-allose, D-altrose and their disaccharides using biotechnology method.
From the result of this research, we succeeded to mass-production of recombinant D-glucoside 3-dehydrogenase and its crystalization. This enzyme showed the substrate specificity against various disaccharides. By combination of chemical hydrogenation, we achieved to produced various rare sugar containing disaccharide such as 1,4-a-allosylglucose, 1,4-b-allosylglucose, 1,1-a-allosylallose, and 1,4-b-gulosylglucose.

Research Products

• [Journal Article] Amylolytic Enzymes Acquired from L-Lactic Acid Producing Enterococcus faecium K-1 and Improvement of Direct Lactic Acid Production from Cassava Starch (2017)
  • Author(s): Kridsada Unban, Apinun Kanpiengjai, Goro Takata, Keiko Uechi, Wen-Chien Lee, and Chartchai Khanongnuch
  • Journal Title: Applied Biochemistry and Biotechnology Volume: in press Issue: 1 Pages: 155-170
  • DOI: 10.1007/s12010-017-2436-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] X-ray-induced mutation of Bacillus sp. MR10 for manno-oligosaccharides production from copra meal (2017)
  • Author(s): Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Pairote Wongputtisin, Goro Takata, and Chartchai Khanongnuch
  • Journal Title: Preparative Biochemistry and Biotechnology Volume: 47 Issue: 4 Pages: 424-433
  • DOI: 10.1080/10826068.2016.1252929
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Essentiality of tetramer formation of Cellulomonas parahominis L-ribose isomerase involved in novel L-ribose metabolic pathway. (2015)
  • Author(s): Yuji Terami, Hiromi Yoshida, Keiko Uechi, Kenji Morimoto, Goro Takata, Shigehiro Kamitori
  • Journal Title: Applied Microbiology and Biotechnology Volume: - Issue: 15 Pages: 6303-6313
  • DOI: 10.1007/s00253-015-6417-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Production and application of a rare disaccharide using sucrose phosphorylase from Leuconostoc mesenteroides (2015)
  • Author(s): Morimoto, K., Yoshihara, A., Furumoto, T., Takata, G.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 119(6) Issue: 6 Pages: 652-656
  • DOI: 10.1016/j.jbiosc.2014.11.011
  • NAID: 110009970753
  • Peer Reviewed
• [Journal Article] Production of L-allose and D-talose from L-psicose and D-tagatose by L-ribose isomerase (2015)
  • Author(s): Terami, Y., Uechi, K., Nomura, S., Okamoto, N., Morimoto, K., Takata, G.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 79(10) Issue: 10 Pages: 1725-1729
  • DOI: 10.1080/09168451.2015.1038215
  • Peer Reviewed
• [Journal Article] Structure of d-tagatose 3-epimerase-like protein from Methanocaldococcus jannaschii (2014)
  • Author(s): Uechi, K., Takata, G., Yoneda, K., Ohshima, T., Sakuraba, H.
  • Journal Title: Acta Crystallographica Section F:Structural Biology Communication Volume: 70 Issue: 7 Pages: 890-895
  • DOI: 10.1107/s2053230x14011005
  • Peer Reviewed / Open Access
• [Journal Article] X-ray structure of a novel L-ribose isomerase acting on a non-natural sugar L-ribose as its ideal substrate. (2014)
  • Author(s): Yoshida H., Yoshihara A., Teraoka M., Terami Y., Takata G., Izumori K., Kamitori S.
  • Journal Title: FEBS J. Volume: 281 Issue: 14 Pages: 3150-3164
  • DOI: 10.1111/febs.12850
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Application of galactokinase for oligosaccharide synthesis (2016)
  • Author(s): Yoshiyasu Sugihara, Goro Takata, Akkharapimon Yotsombat, and Chartchai Khanongnuch
  • Organizer: Core to core program A. Advanced research networks
  • Place of Presentation: Chonburi, Thailand
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Amylase from L-lactic acid producing amylolytic Enterococcus faecium K-1 and its influence on direct lactic acid fermentation from starchy substrate (2016)
  • Author(s): Chartchai Khanongnuch, Krisada Unban, Goro Takata, and Keiko Uechi
  • Organizer: Core to core program A. Advanced research networks
  • Place of Presentation: Chonburi, Thailand
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Recombinant expression and characterization of cellobiose 2-epimerase from Enterococcus phoeniculicola (2016)
  • Author(s): Kohei Mino, Keiko Uechi, Goro Takata, Akkharapimon Yotsombat, Yuji Terami
  • Organizer: Core to core program A. Advanced research networks
  • Place of Presentation: Chonburi, Thailand
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Regulation analysis of sugar metabolic pathway by genome editing method (2016)
  • Author(s): Akane Terai, Goro Takata, Akkharapimon Yotsombat,Yuji Terami
  • Organizer: Core to core program A. Advanced research networks
  • Place of Presentation: Chonburi, Thailand
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Functional modification of cellobiose phosphorylase from Clostridium thermocellum (2016)
  • Author(s): Mami Kazama, Goro Takata, Akkharapimon Yotsombat,Yuji Terami
  • Organizer: Core to core program A. Advanced research networks
  • Place of Presentation: Chonburi, Thailand
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] 酵素法を用いた希少糖生産の新展開 (2015)
  • Author(s): 高田悟郎
  • Organizer: 平成27年度日本応用糖質科学会中国･四国支部シンポジウム
  • Place of Presentation: 福山
  • Year and Date: 2015-11-20
  • Invited
• [Presentation] Purification and characterization of the D-glucoside 3-dehydrogenase from m15-2, (2015)
  • Author(s): Akkharapimon Yotsombat, Goro Takata, Keiko Uechi, Yuji Terami.
  • Organizer: International symposium on microbial research and biotechnology for biomass utilization
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Structural insight into L-rhamnose isomerase from Mesorhizobium loti (2015)
  • Author(s): Mami Kazama, Keiko Uechi, Yuji Terami, Haruhiko Sakuraba, Goro Takata
  • Organizer: International symposium on microbial research and biotechnology for biomass utilization
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Elucidation of functional sugar production using enzyme and fermentation technology of microorgasms (2015)
  • Author(s): Goro Takata, Keiko Uechi, Yuji Terami, Chartchai Khanongnuch, Akane Terai, Mami Kazama, Kohei Nawa, Akkharapimon Yotsombat
  • Organizer: International symposium on microbial research and biotechnology for biomass utilization
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-13
  • Int'l Joint Research / Invited
• [Presentation] Screening of nigeran hydrolyzing microorganism (2015)
  • Author(s): Akane Terai, Goro Takata, Keiko Uechi, Yuji Terami
  • Organizer: International symposium on microbial research and biotechnology for biomass utilization
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Cellulomonas parahominis由来L-リボースイソメラーゼの基質複合体構造解析および四量体形成の必要性の解明 (2015)
  • Author(s): 寺見優司，上地敬子，森本兼司，吉田裕美，神鳥成弘，髙田悟郎
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Rhizobium sp. S10株由来のD-グルコシド-3-デヒドロゲナーゼによるゲンチオビオースから新規二糖の生産 (2015)
  • Author(s): 野村彩希，上地敬子，寺見優司，髙田悟郎.
  • Organizer: 日本農芸化学会
  • Place of Presentation: 水産大学校
  • Year and Date: 2015-01-21
• [Presentation] 根粒菌由来のD-グルコシド3-デヒドロゲナーゼの精製と諸性質の検討 (2014)
  • Author(s): 野村彩希, 上地敬子, 寺見優司, 森本兼司, 高田悟郎
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-07 – 2014-09-09
• [Presentation] Geodermatophilus obscurus NBRC13315由来L-リボースイソメラーゼの性質と構造解析 (2014)
  • Author(s): 寺見優司，上地敬子，髙田悟郎，吉田裕美，神鳥成弘.
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-07 – 2014-09-09
• [Presentation] D-グルコシド3-デヒドロゲナーゼを用いた3-ケトセロビオースの生産 (2014)
  • Author(s): 野村彩希，上地敬子，寺見優司，髙田悟郎
  • Organizer: 第28回セルラーゼ研究会
  • Place of Presentation: 幕張セミナーハウス
  • Year and Date: 2014-07-10 – 2014-07-11