| Project/Area Number |
26450097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kagawa University |
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Principal Investigator |
Takata Goro 香川大学, 国際希少糖研究教育機構, 准教授 (50322722)
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| Co-Investigator(Kenkyū-buntansha) |
森本 兼司 香川大学, 国際希少糖研究教育機構, 准教授 (90363184)
上地 敬子 香川大学, 希少糖研究センター, 博士研究員 (70733426)
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| Co-Investigator(Renkei-kenkyūsha) |
Sakuraba Haruhiko 香川大学, 農学部, 准教授 (90205823)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 希少糖 / D-アロース / 希少二糖 / D-グルコシド3-デヒドロゲナーゼ |
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| Outline of Final Research Achievements |
The objective of this study is the production of D-allose, D-altrose and their disaccharides using biotechnology method.
From the result of this research, we succeeded to mass-production of recombinant D-glucoside 3-dehydrogenase and its crystalization. This enzyme showed the substrate specificity against various disaccharides. By combination of chemical hydrogenation, we achieved to produced various rare sugar containing disaccharide such as 1,4-a-allosylglucose, 1,4-b-allosylglucose, 1,1-a-allosylallose, and 1,4-b-gulosylglucose.
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