Regulation of nitrogen fixation in actinobacteria
Project/Area Number |
26450098
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Kagoshima University |
Principal Investigator |
KUCHO Ken-ichi 鹿児島大学, 理工学域理学系, 准教授 (70404473)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 順遺伝学 / 突然変異株 / ゲノム解析 / 窒素固定 / 放線菌 / 酸素防御 / 転写制御 / 突然変異体 / 遺伝子発現 / ゲルシフト / 共生 / アンモニア / DNA分解酵素 / 形質転換 |
Outline of Final Research Achievements |
Frankia spp. are nitrogen-fixing actinobacteria and have several unique properties. Frankia develops vesicles, which are spherical multicellular structure devoted to nitrogen fixation. Frankia genomes do not contain regulatory genes for nitrogen-fixation (nif) genes that are typically found in other nitrogen-fixing bacteria. We isolated 49 Frankia mutants that cannot fix nitrogen. These mutants included those showed aberrant vesicle phenotypes (less number, smaller size, thinner envelope) and reduced nif gene expression. They are considered to carry mutations in genes involved in vesicle differentiation and nif gene regulation. We analyzed genomes of mutants and revertants, and specified a gene that is responsible for the mutant phenotype.
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Report
(5 results)
Research Products
(15 results)
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[Presentation] 山崩れによりかく乱された御嶽山における根粒と根圏土壌中のフランキアの群集構造2016
Author(s)
池邉茉莉,中島沙映,山元巧,柴田銃江,今矢明宏,壁谷大介,齋藤智之,岡本透,小野賢治,森貞和仁,飛田博順,九町 健一
Organizer
日本微生物生態学会第31回大会
Place of Presentation
横須賀市文化会館(神奈川県横須賀市)
Year and Date
2016-10-22
Related Report
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