| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Frankia spp. are nitrogen-fixing actinobacteria and have several unique properties. Frankia develops vesicles, which are spherical multicellular structure devoted to nitrogen fixation. Frankia genomes do not contain regulatory genes for nitrogen-fixation (nif) genes that are typically found in other nitrogen-fixing bacteria.
We isolated 49 Frankia mutants that cannot fix nitrogen. These mutants included those showed aberrant vesicle phenotypes (less number, smaller size, thinner envelope) and reduced nif gene expression. They are considered to carry mutations in genes involved in vesicle differentiation and nif gene regulation. We analyzed genomes of mutants and revertants, and specified a gene that is responsible for the mutant phenotype.
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