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The regulatory mechanisms of the induction of lipase expression and polyester production by stearyl alcohol

Research Project

Project/Area Number 26450101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionRikkyo University (2015-2016)
Chuo University (2014)

Principal Investigator

AKANUMA Genki  立教大学, 理学部, 助教 (30580063)

Co-Investigator(Kenkyū-buntansha) 石塚 盛雄  中央大学, 理工学部, 教授 (50168241)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリパーゼ / ポリエステル / Ralstonia / Lipase / PHB / Ralstonia / Lipase / EliA / PHB
Outline of Final Research Achievements

The transcriptional factor which activates the transcription of gene encoding lipase was determined. Moreover, we found the alcohol dehydrogenase which plays important role for induction of lipase expression by stearyl alcohol. The EliA, a secretory protein, is essential for the lipase-producing bacterium to respond to stearyl alcohol.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] EliA is required for inducing the stearyl alcohol-mediated expression of secretory proteins and production of polyester in Ralstonia sp. NT80.2016

    • Author(s)
      Akanuma G, Yoshizawa R, Nagakura M, Shiwa Y, Watanabe S, Yoshikawa H, Ushio K, Ishizuka M.
    • Journal Title

      Microbiology

      Volume: 162 Issue: 2 Pages: 408-419

    • DOI

      10.1099/mic.0.000225

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis.2016

    • Author(s)
      Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, Hanai R, Nanamiya H, Kato-Yamada Y, Kawamura F.
    • Journal Title

      Microbiology

      Volume: 162 Issue: 3 Pages: 448-458

    • DOI

      10.1099/mic.0.000234

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.2014

    • Author(s)
      Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M.
    • Journal Title

      Journal of Bacteriology

      Volume: 196 Issue: 22 Pages: 3820-3830

    • DOI

      10.1128/jb.01896-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Ralstonia sp. NT-80 株におけるリパーゼ誘導促進因子の機能解析2016

    • Author(s)
      千濱良太、石橋隼、粟井貴子、赤沼元気、牛尾一利、石塚盛雄
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ralstonia sp. NT-80株におけるリパーゼ 転写調節因子LipR80-4の機能解析2016

    • Author(s)
      多勢 真大、関谷 麻美、赤沼 元気、石塚 盛雄
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Ralstonia sp.NT-80株におけるリパーゼ転写調節因子LipR80-4の機能解析2015

    • Author(s)
      多勢 真大、関谷 麻美、赤沼 元気、石塚 盛雄
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 高級アルコールに対する微生物の反応2014

    • Author(s)
      赤沼 元気、吉澤 梨絵、永倉 茉莉、大塚 拓、志波 優、渡辺 智、吉川 博文、牛尾 一利、石塚 盛雄
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      山形県鶴岡市
    • Year and Date
      2014-09-03 – 2014-09-05
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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