Increasing radioresistance of the extreme thermophile by introducing a gene from the radioresistant bacterium
| Project/Area Number |
26450103
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Toyo University |
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Principal Investigator |
Narumi Issay 東洋大学, 生命科学部, 教授 (90343920)
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| Co-Investigator(Renkei-kenkyūsha) |
SATOH Katsuya 国立研究開発法人量子科学技術研究開発機構, 量子ビーム科学研究部門, 上席研究員 (90370402)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DNA修復 / 放射線抵抗性細菌 / 高度好熱菌 / 遺伝子発現 / タンパク質改変 |
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| Outline of Final Research Achievements |
DNA repair promoting protein PprA derived from the moderately thermophilic radioresistant bacterium Deinococcus geothermalis was expressed in the extremely thermophilic bacterium Thermus thermophilus. The PprA-expressed T. thermophilus strain showed an increased resistance to hydrogen peroxide and ethyl methanesulfonate, suggesting that PprA protein is involved in the repair of DNA oxidative and alkylation damage. This study revealed that the improvement of DNA repair function is possible by expressing a DNA repair promoting protein specific to the radioresistant bacterium in an extreme thermophile.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Draft Genome Sequence of Bacillus alcalophilus AV1934, a Classic Alkaliphile Isolated from Human Feces in 1934.2014
Author(s)
Attie O, Jayaprakash A, Shah H, Paulsen IT, Morino M, Takahashi Y, Narumi I, Sachidanandam R, Satoh K, Ito M, and Krulwichc TA.
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Journal Title
Genome Announcements
Volume: 2(6)
Issue: 6
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Current status on space exposure and capture experiments of microbes in TANPOPO mission at International Space Station2015
Author(s)
Shin-ichi Yokobori, Yuko Kawaguchi, Kaori Tomita-Yokotani, Nobuhiro Hayashi, Issay Narumi, Makoto Tabata, Hideyuki Kawai, Kyoko Okudaira, Yoko Kebukawa, Kensei Kobayashi, Hajime Mita, Kazumichi Nakagawa, Hikaru Yabuta, Eiichi Imai, Satoshi Sasaki, Masumi Higashide, Hirofumi Hashimoto, Hajime Yano, Akihiko Yamagishi
Organizer
第8回アストロバイオロジーワークショップ
Place of Presentation
東京工業大学（東京都目黒区）
Year and Date
2015-11-27
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[Presentation] Space exposure experiment of microbes at the international space station proposed in “Tanpopo” mission2014
Author(s)
Shin-ichi Yokobori, Yuko Kawaguchi, Yinjie Yang, Hirofumi Hashimoto, Nobuhiro Hayashi, Kensei Kobayashi, Kazumichi Nakagawa, Issay Narumi, Kaori Tomita-Yokotani, Satoshi Yoshida, Hajime Yano, Akihiko Yamagishi
Organizer
The Asia Oceania Geosciences Society 11th Annual Meeting (AOGS 2014)
Place of Presentation
ロイトン札幌（北海道札幌市）
Year and Date
2014-07-28 – 2014-08-01
Related Report
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[Presentation] The possible interplanetary transfer of microbes: Assessing the viability of Deinococcus spp. under the ISS environmental conditions for performing exposure experiments of microbes in the Tanpopo mission2014
Author(s)
Yuko Kawaguchi, Yinjie Yang, Narutoshi Kawashiri, Keisuke Shiraishi, Masako Takasu, Issay Narumi, Katsuya Satoh, Hirofumi Hashimoto, Kazumichi Nakagawa, Yoshiaki Tanigawa, Yoh-hei Momoki, Maiko Tanabe, Tomohiro Sugino, Yuta Takahashi, Yasuyuki Shimizu, Satoshi Yoshida, Kensei Kobayashi, Shin-ichi Yokobori, et al.
Organizer
Origins 2014 International Conference
Place of Presentation
奈良県新公会堂（奈良県奈良市）
Year and Date
2014-07-06 – 2014-07-11
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