• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Augmentation of dietary sphingolipids utilization with the novel intestinal bacterium and its application to the alleviation or prevention of colon diseases

Research Project

Project/Area Number 26450104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionMeiji University

Principal Investigator

Asanuma Narito  明治大学, 農学部, 専任准教授 (50366902)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsスフィンゴ脂質 / 腸内細菌 / プロバイオティクス / セラミド / グルコシルセラミド / 炎症性腸疾患
Outline of Final Research Achievements

A novel anaerobic bacterium capable of hydrolyzing plant glucosylceramide (GluCer) was isolated from canine feces. This isolate hydrolyzed GluCer to ceramide without producing sphingoids at a higher rate than other bacteria. This enables the production of large amounts of ceramide. Oral administration of the isolate to mice fed diets supplemented with GluCer enhanced GluCer digestion without showing acute toxicity. Oral administration of the isolate with dietary GluCer to dextran sodium sulfate (DSS)-treated mice (inflammatory bowel disease model) alleviated the symptoms of colitis including body weight loss, diarrhea, and bloody stool. Myeloperoxidase (MPO) activity levels in colonic tissue were increased greatly in response to DSS, but probiotic utilization of this isolate lowered MPO activity significantly. Thus, this novel isolate might be used as a probiotic to augment the bioactive effects of dietary GluCer without the adverse effects.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 7 results)

  • [Journal Article] 食事性グルコシルセラミドによる皮膚バリア機能の改善と大腸炎の緩和・抑制2016

    • Author(s)
      川田実生, 浅沼成人
    • Journal Title

      明治大学農学部研究報告

      Volume: 65 Pages: 83-93

    • NAID

      120005941791

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of nitrate addition to a diet on fermentation and microbial populations in the rumen of goats, with special reference to Selenomonas ruminantium having the ability to reduce nitrate and nitrite2015

    • Author(s)
      Asanuma N, Yokoyama S, Hino T
    • Journal Title

      Animal Science Journal

      Volume: 86 Issue: 4 Pages: 378-384

    • DOI

      10.1111/asj.12307

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Characterizations of plant glucosylceramide hydrolyzing bacteria isolated from canine feces2016

    • Author(s)
      Kawata M, Tsukamoto A, Asanuma N
    • Organizer
      16th International Nutrition & Diagnostics Conference (INDC2016)
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Augmentation of the utilization of dietary glucosylcermide by the novel Bacterium A2016

    • Author(s)
      Tsukamoto A, Kawata M, Asanuma N
    • Organizer
      16th International Nutrition & Diagnostics Conference (INDC2016)
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inhibition of melanogenesis by plant ceramide in B16 melanoma cells2016

    • Author(s)
      Akutsu S, Kawahara N, Asanuma N
    • Organizer
      16th International Nutrition & Diagnostics Conference (INDC2016)
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Availability of dog feces as the amino acid source for Butyrivibrio fibrisolvens2016

    • Author(s)
      Kawahara N, Akutsu S, Asanuma N
    • Organizer
      16th International Nutrition & Diagnostics Conference (INDC2016)
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of plant ceramide as antibacterial agent to prevent Escherichia coli O157:H7 infection2016

    • Author(s)
      Asanuma N
    • Organizer
      IV International Conference on Antimicrobial Research (ICAR2016)
    • Place of Presentation
      Torremolinos-Malaga, Spain
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Immunomodulatory effects of lipopolysaccharide from the Butyrivibrio group bacterium isolated from the goat rumen2015

    • Author(s)
      Asanuma N
    • Organizer
      15th International Nutrition & Diagnostics Conference
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-10-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of oral administration of the Butyrivibrio group bacterium on the inflammatory dry skin condition in chronic irritant contact dermatitis2015

    • Author(s)
      Asanuma N
    • Organizer
      6th Congress of European Microbiologists
    • Place of Presentation
      Maastricht, The Netherlands
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Probiotic utilization of the novel glucosylceramide-hydrolyzing bacterium to the alleviation of inflammatory bowel disease2014

    • Author(s)
      Asanuma N
    • Organizer
      14th International Nutrition & Diagnostics Conference
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Probiotic Effects of the Newly Ceramide-Producing Bacterium with Dietary Supplementation of Glucosylcermide2014

    • Author(s)
      Asanuma N, Tsukamoto A, Yamaya M
    • Organizer
      International Scientific Conference on Probiotics and Prebiotics -IPC2014
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2014-06-24 – 2014-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Application of Plant Ceramide to Modulate Intestinal Microflora2014

    • Author(s)
      Yokoyama S, Asanuma N
    • Organizer
      International Scientific Conference on Probiotics and Prebiotics -IPC2014
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2014-06-24 – 2014-06-26
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi