Function analysis of galactofuranosyltransferases in filamentous fungus for development of novel antifungal agent
Project/Area Number |
26450106
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Sojo University |
Principal Investigator |
OKA Takuji 崇城大学, 生物生命学部, 准教授 (50510690)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | ガラクトマンナン / ガラクトフラノース / 糖鎖 / 糖転移酵素 / 糸状菌 / 細胞壁 / 糖鎖生合成 / Aspergillus gumigatus / 糖鎖生合性 / Aspergillus fumigatus / 肺アスペルギルス症 / 抗真菌剤 |
Outline of Final Research Achievements |
The galactomannans (GMs) that are produced by filamentous fungi belonging to Pezizomycotina, many of which are pathogenic for animals and plants, are polysaccharides consisting of α-(1→2)-/α-(1→6)-mannosyl and β-(1→5)-/β-(1→6)-galactofuranosyl residues. GMs are located at the outermost layer of the cell wall. When a pathogenic fungus infects a host, its cell surface must be in contact with the host. The GMs on the cell surface may be involved in the infection mechanism of a pathogenic fungus or the defense mechanism of a host. There are two types of GMs in filamentous fungi, fungal-type galactomannans and O-mannose type galactomannans. In this study, we elucidated that AfGfsA protein acts as a UDP-Galf: β-galactofuranoside β1,5-galactofuranosyltransferase and all β-(1→5)-galactofuranosyl residues of FTGM and OMGM in A. fumigatus were biosynthesized by AfGfsA and AfGfsC.
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Report
(5 results)
Research Products
(36 results)
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[Journal Article] Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans.2014
Author(s)
Oka, T., Katafuchi, Y., Fukuda, K., Ekino, K., Goto, M. and Nomura, Y.
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans.2014
Author(s)
Oka, T., Katafuchi, Y., Fukuda, K., Ekino, K., Goto, M. and Nomura, Y.
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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