Transcriptional regulation machinery of conidia specific hydrophilin

• Project/Area Number: 26450108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: SUZUKI Satoshi 国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門 食品生物機能開発研究領域, 上級研究員 (90353979)
• Co-Investigator(Renkei-kenkyūsha): KUSUMOTO Ken-Ichi 国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門 食品生物機能開発研究領域, 食品醸造微生物ユニット長 (80353978)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Aspergillus / 光応答 / 糸状菌光応答 / 分生子タンパク質 / 糸状菌 / hydrophilin / Light / con gene / conidia

Outline of Final Research Achievements

In order to identify the transcription factor complex that binds to cis element of light regulation factor on 5’ UTR of hydrophilin gene, following experiments were carried out. The nuclear extract of Aspergillus nidulans and biotinylated DNA probe of the cis element of light regulation factor of 5’ UTR of hydrophilin gene were mixed and co-precipitated with streptavidin magnetic beads. Then the transcription factor complex were fragmented by tryptic digestion and subjected to MS analysis.

Research Products

• [Int'l Joint Research] メイヌース大学(アイルランド)