Production and purification of fatty acid contributing to prevention of atopic dermatitis through microbial conversion and enzymatic process.
| Project/Area Number |
26450113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka Research Institute of Industrial Science and Technology (2017)
Osaka Municipal Technical Research Institute (2014-2016) |
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Principal Investigator |
Nagao Toshihiro 地方独立行政法人大阪産業技術研究所, 森之宮センター, 研究室長 (30416309)
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| Co-Investigator(Kenkyū-buntansha) |
田中 重光 地方独立行政法人大阪産業技術研究所, 生物・生活材料研究部, 研究員 (20509822)
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| Research Collaborator |
KISHIMOTO Noriaki 近畿大学, 農学研究院応用生物科学科, 教授
KURATA Atsushi 近畿大学, 農学研究院応用生物科学科, 准教授
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Staphylococcus aureus / S. epidermidis / アトピー性皮膚炎 / パルミトレイン酸 / 皮脂 / 抗菌活性 / 菌叢 / スキンマイクロバイオーム / パルミトオレイン酸 |
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| Outline of Final Research Achievements |
Skin microbiota is concerned in several diseases and healthy condition, we thus focus on Staphylococcus aureus and S. epidermidis.
Aeromonas hydrophila converted 9cis-C18:1 to 7cis-C16:1 and 5cis-C14:1. 7cis-C16:1 showed selective anti-bacterial activity which suppressed growth of S. aureus and did not suppress growth of S. epidermidis. So, we attempted purification of 7cis-C16:1. Purification of 7cis-C16:1 was not succeeded through enzymatic process, but ODS column was effective for purification of 7cis-C16:1 (86% purity).
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Report
(5 results)
Research Products
(29 results)
