| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of this study is to make donor substrates for transglycosylation, which glycosidases catalyze. Using β-fructofranosidase and levansucrase, I succeeded in transferring a fructosyl group of sucrose to each saccharide, such as xylose, galactose, mannose, isomaltooligosaccharides, maltooligosaccharides, and glucuronic acid. The reaction product can be a donor substrate of the transglycosylation by glycosidases. Furthermore, I investigated the transglycosylation properties of several glycosidases and modified their specificity through molecular analyses. In addition, nucleotide sugar was successfully synthesized by the reaction of sucrose synthase with fructosyl saccharide as substrate.
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