| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this research the molecular mechanism of oxidative stress response by human cell are analyzed. In order to unveil the molecular mechanism in which the sensor protein Keap1 recognize the oxidative stress and various toxic chemicals, I prepared the recombinant Keap1 protein for the crystal analysis. The crystallization of Keap1 mutants was succeeded, and X-ray diffraction analysis was performed by using the synchrotron X-ray source performed X-ray diffraction experiment. The method to detect the intercalations between Keap1 and the toxic chemicals from vegetables was developed.
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