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Elucidation of the reaction mechanism of plant flavonoid C-glucosyltransferases

Research Project

Project/Area Number 26450120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionShinshu University

Principal Investigator

TAGUCHI Goro  信州大学, 学術研究院繊維学系, 准教授 (70252070)

Co-Investigator(Kenkyū-buntansha) 新井 亮一  信州大学, 学術研究院繊維学系, 准教授 (50344023)
鈴木 秀幸  公益財団法人かずさDNA研究所, バイオ研究開発部, グループ長 (80276162)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsC-配糖化酵素 / フラボノイドC-配糖体 / 酵素反応解析 / X線結晶構造解析 / フラボノイド C-配糖体 / フラボノイド-C-配糖体 / 結晶構造解析 / 配糖体生産 / 生物変換
Outline of Final Research Achievements

In this project, we identified C-glycosyltransferases those are responsible for the biosynthesis of valuable flavonoid C-glycosides from several plant species, such as buckwheat, citrus and leguminous plants. We characterized the enzymatic reaction of them precisely, and also analyzed the crystal structure of the protein to elucidate the reaction mechanisms of the C-glycosyltransferases producing carbon-carbon bonds between the sugar moiety and the aromatic carbon of flavonoids. We also achieved the production of C-glucosides of flavonoid by bioconversion using Escherichia coli expressing C-glucosyltransferases.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants.2017

    • Author(s)
      Ito T, Fujimoto S, Suito F, Shimosaka M, Taguchi G.
    • Journal Title

      Plant Journal

      Volume: 印刷中 Issue: 2 Pages: 187-198

    • DOI

      10.1111/tpj.13555

    • NAID

      120007099971

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.2014

    • Author(s)
      Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.
    • Journal Title

      Plant Journal

      Volume: 80 Issue: 3 Pages: 437-448

    • DOI

      10.1111/tpj.12645

    • NAID

      120007100762

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Production of <i>C</i>-glucosides of flavonoids and related compounds by <i>Escherichia coli</i> expressing buckwheat <i>C</i>-glucosyltransferase2014

    • Author(s)
      Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 5 Pages: 519-524

    • DOI

      10.5511/plantbiotechnology.14.1016a

    • NAID

      130004721170

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ソバ由来フラボノイドC-配糖化酵素の反応メカニズムの調査2017

    • Author(s)
      小柳祐輔、水藤史明、新井亮一、田口悟朗
    • Organizer
      日本農芸化学会2017年度大会(京都)大会
    • Place of Presentation
      京都女子大学(京都市東山区)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] ワサビ(Wasabia japonica)のイソサポナリン生合成に関与する C-配糖化酵素の同定2017

    • Author(s)
      眞島京子,鈴木秀幸,田口悟朗
    • Organizer
      日本農芸化学会2017年度大会(京都)大会
    • Place of Presentation
      京都女子大学(京都市東山区)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] ソバ由来フラボノイドC-配糖化酵素のX線結晶構造解析2016

    • Author(s)
      小柳祐輔、水藤史明、新井亮一、田口悟朗
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学繊維学部(長野県上田市)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] ワサビのイソサポナリン生合成に関わるC-配糖化酵素の探索2016

    • Author(s)
      眞島京子,鈴木秀幸,田口悟朗
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学繊維学部(長野県上田市)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] タルウマゴヤシ(Medicago truncatula)由来フラボノイド C-配糖化酵素の機能解析2016

    • Author(s)
      小柳祐輔、伊藤崇充、山本菜摘、田口悟朗
    • Organizer
      日本農芸化学会2016年度大会(札幌)
    • Place of Presentation
      札幌コンベンションセンター(札幌市白石区)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] ダイズ(Glycine max)のフラボノイドC 配糖化酵素の機能解析2015

    • Author(s)
      小柳祐輔、山本菜摘、内田 開、明石智義、田口悟朗
    • Organizer
      第33回日本植物細胞分子生物学会(東京)大会・シンポジウム
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-08-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物の配糖体と配糖化酵素2015

    • Author(s)
      田口悟朗
    • Organizer
      平成27年度日本応用糖質科学会東日本支部シンポジウム 『広がる糖質科学の世界』-配糖体・多糖類・関連酵素-
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] キュウリ(Cucumis sativus)における、フラボノイドC配糖化酵素遺伝子の探索と解析2015

    • Author(s)
      藤本俊介、伊藤崇充、下坂誠、田口悟朗
    • Organizer
      日本農芸化学会2015年度大会(岡山)
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] ミカン科植物のフラボノイドC配糖化酵素の解析2014

    • Author(s)
      伊藤崇充、藤本俊介、小柳祐輔、下坂誠、田口悟朗
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      アイーナ(岩手県盛岡市)
    • Year and Date
      2014-08-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 植物の配糖化酵素遺伝子を発現させた大腸菌による配糖体の生産2014

    • Author(s)
      伊藤崇充、藤本俊介、小柳祐輔、下坂誠、田口悟朗
    • Organizer
      日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      アイーナ(岩手県盛岡市)
    • Year and Date
      2014-08-21
    • Related Report
      2014 Research-status Report
  • [Book] Molecular Breeding and Nutritional Aspects of Buckwheat(担当部分のタイトル Chapter 30: Flavonoid Biosynthesis in Buckwheat)2016

    • Author(s)
      Zhou M., Kreft I., Woo SH., Chrungoo N., Wieslander G. 編、Ohnishi O., Taguchi G ら計83名で分担執筆。 担当章 (Chapter 30) の著者 Taguchi G.)
    • Total Pages
      459
    • Publisher
      Academic Press (Elsevier)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 信州大学繊維学部 田口研究室

    • URL

      http://fiber.shinshu-u.ac.jp/taguchi/index.html

    • Related Report
      2016 Annual Research Report 2014 Research-status Report
  • [Remarks] 信州大学繊維学部 田口研究室HP

    • URL

      http://fiber.shinshu-u.ac.jp/taguchi/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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