| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this project, we identified C-glycosyltransferases those are responsible for the biosynthesis of valuable flavonoid C-glycosides from several plant species, such as buckwheat, citrus and leguminous plants. We characterized the enzymatic reaction of them precisely, and also analyzed the crystal structure of the protein to elucidate the reaction mechanisms of the C-glycosyltransferases producing carbon-carbon bonds between the sugar moiety and the aromatic carbon of flavonoids. We also achieved the production of C-glucosides of flavonoid by bioconversion using Escherichia coli expressing C-glucosyltransferases.
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