| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We investigated whether site-1 protease (S1P) is responsible for cleaving (pro)renin receptor (PRR) to generate soluble PRR. Reduction of endogenous S1P with siRNA attenuated soluble PRR generation in CHO cells exogenously expressing human PRR; conversely, overexpression of S1P by transient transfection increased soluble PRR generation. The S1P inhibitor PF429242 suppressed soluble PRR generation in CHO and HeLa cells; however, the ADAM inhibitor GM6001 had no effect. Moreover, furin inhibitor I had no effect on the amount of soluble PRR, but caused an increase in its size. Taken together, these results suggest that soluble PRR is generated by sequential processing by S1P and furin.
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