| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Biosynthesis of triterpenoids is strictly regulated, as it is often limited to specific organs or tissues, or induced in response to external stimuli. However, the regulatory mechanism of triterpenoid biosynthesis is largely unknown. Glycyrrhizin is a triterpenoid saponin produced by Glycyrrhiza species (licorice) that is widely used as a medicine and natural sweetener. In addition to glycyrrhizin, licorice also produces other types of triterpenoids, such as soyasaponins. To elucidate the regulatory mechanisms behind tissue-type- and stimuli-dependent triterpenoid metabolism in licorice, we aimed to identify transcription factors (TFs) regulating the expression of triterpenoid biosynthetic genes. As a result, we identified a TF able to transactivate the CYP93E3 promoter by approximately 30-fold to promote soyasaponins biosynthesis.
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