Regulatory mechanisms of triterpenoid biosynthesis in plants
Project/Area Number |
26450123
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Osaka University |
Principal Investigator |
SEKI HIKARU 大阪大学, 工学研究科, 准教授 (30392004)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 植物トリテルペノイド / 生合成 / プロモーター / 転写因子 / シトクロムP450モノオキシゲナーゼ / P450 / 植物 / トリテルペン / 転写制御因子 / トリテルペノイド / 生合成酵素遺伝子 / 発現制御 |
Outline of Final Research Achievements |
Biosynthesis of triterpenoids is strictly regulated, as it is often limited to specific organs or tissues, or induced in response to external stimuli. However, the regulatory mechanism of triterpenoid biosynthesis is largely unknown. Glycyrrhizin is a triterpenoid saponin produced by Glycyrrhiza species (licorice) that is widely used as a medicine and natural sweetener. In addition to glycyrrhizin, licorice also produces other types of triterpenoids, such as soyasaponins. To elucidate the regulatory mechanisms behind tissue-type- and stimuli-dependent triterpenoid metabolism in licorice, we aimed to identify transcription factors (TFs) regulating the expression of triterpenoid biosynthetic genes. As a result, we identified a TF able to transactivate the CYP93E3 promoter by approximately 30-fold to promote soyasaponins biosynthesis.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Cytochrome P450 monooxygenase CYP716A141 is a unique β-amyrin C-16β oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus.2017
Author(s)
Tamura, K., Teranishi, Y., Ueda, S., Suzuki, H., Kawano, N., Yoshimatsu, K., Saito, K., Kawahara, N., Muranaka, T. and Seki, H.
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Journal Title
Plant Cell Physiol.
Volume: 印刷中
Issue: 5
Pages: 874-884
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.2017
Author(s)
Tamura, K., Seki, H., Suzuki, H., Kojoma, M., Saito, K. and Muranaka, T.: CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.
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Journal Title
Plant Cell Rep.
Volume: 36
Issue: 3
Pages: 437-445
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Molecular cloning and characterization of triterpene oxidases in Platycodon grandifloras.2017
Author(s)
Tamura, K., Teranishi, Y., Suzuki, H., Kawano, N., Yoshimatsu, K., Saito, S., Kawahara, N., Muranaka, T. and Seki, H.
Organizer
第58回日本植物生理学会年会
Place of Presentation
鹿児島大学郡元キャンパス(鹿児島市)
Year and Date
2017-03-16
Related Report
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[Presentation] Exploration of the transcription factors regulating triterpenoid biosynthesis in licorice.2017
Author(s)
Yoshida, K., Tamura, K., Seki, H., Hiraoka, Y., Mochida, K., Suzuki, H., Kojoma, M., Mitsuda, N., Saito, K. and Muranaka, T.
Organizer
第58回日本植物生理学会年会
Place of Presentation
鹿児島大学郡元キャンパス(鹿児島市)
Year and Date
2017-03-16
Related Report
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[Presentation] Identification of β-amyrin 28-oxidase in Glycyrrhiza uralensis.2016
Author(s)
Tamura, K., Seki, H., Suzuki, H., Kojoma, M. and Muranaka, T.
Organizer
55th Annual Meeting of the Phytochemical Society of North America
Place of Presentation
Davis, California, USA
Year and Date
2016-08-06
Related Report
Int'l Joint Research
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