| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The aim of this study is to understand the relationship between structural diversity and biological function of complex sphingolipids in yeast Saccharomyces cerevisiae. Results of the research was the followings: (1) it was found that MIPC, one of the subtypes of complex sphingolipid in yeast, is important for maintenance of functions of cell wall, (2) change in activity of serine palmitoyltransferasae affects sensitivity to Syringomycin E, a toxin produced by the plant phathogenic bacterium, (3) downregulation of de novo sphingolipid biosynthesis rescues functional defects caused by deletion of RVS167 and RVS161 encoding the amphiphysin family proteins, (4) deletion of vacuolar H+-ATPase (V-ATPase) causes dramatic alteration of complex sphingolipid composition, and it was suggested that the alteration is an adaptation mechanism for defect of intracellular pH homeostasis.
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