Study on mechanisms of metabolic regulation and structure-function relationship of sphingolipids in budding yeast
| Project/Area Number |
26450127
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Tani Motohiro 九州大学, 理学研究院, 准教授 (20452740)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | スフィンゴ脂質 / 複合スフィンゴ脂質 / セラミド / 出芽酵母 / ストレス応答 |
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| Outline of Final Research Achievements |
The aim of this study is to understand the relationship between structural diversity and biological function of complex sphingolipids in yeast Saccharomyces cerevisiae. Results of the research was the followings: (1) it was found that MIPC, one of the subtypes of complex sphingolipid in yeast, is important for maintenance of functions of cell wall, (2) change in activity of serine palmitoyltransferasae affects sensitivity to Syringomycin E, a toxin produced by the plant phathogenic bacterium, (3) downregulation of de novo sphingolipid biosynthesis rescues functional defects caused by deletion of RVS167 and RVS161 encoding the amphiphysin family proteins, (4) deletion of vacuolar H+-ATPase (V-ATPase) causes dramatic alteration of complex sphingolipid composition, and it was suggested that the alteration is an adaptation mechanism for defect of intracellular pH homeostasis.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling2015
Author(s)
Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K
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Journal Title
EMBO Journal
Volume: 34
Issue: 21
Pages: 2703-2719
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Loss of hydroxyl groups from the ceramide moiety can modify the lateral diffusion of membrane proteins in S. cerevisiae.2014
Author(s)
Uemura, S., Shishido, F., Tani, M., Mochizuki, T., Abe, F., and Inokuchi, J.
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Journal Title
J. Lipid Res.
Volume: 55
Issue: 7
Pages: 1343-1356
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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