| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The mechanisms of the action of lectins were analyzed in detail by the protein engineering techniques, and the novel proteins with new useful functions were developed based on the findings obtained there. In this study, lectins from the marine invertebrates were used. Their entire sequences were determined by the cDNA cloning, and the novel three-dimensional structures were elucidated by X-ray crystallographic analysis. In addition to expressing these recombinant proteins using E. coli cells, we elucidated the role of individual amino acid residues using genetic recombination techniques and successfully developed lectins having novel activities.
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