MRI analysis of olfactory neurocircuits that discriminate odor and pheromone signals

• Project/Area Number: 26450129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kumamoto University
• Principal Investigator: Yoshinaga Sosuke 熊本大学, 大学院生命科学研究部(薬), 講師 (00448515)
• Co-Investigator(Renkei-kenkyūsha): TERASAWA Hiroaki 熊本大学, 大学院生命科学研究部(薬), 教授 (10300956) ; TOUHARA Kazushige 東京大学, 大学院農学生命科学研究科, 教授 (00280925)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 匂い / フェロモン / 脳・神経 / ＭＲＩ / 嗅覚 / 嗅球 / MRI / 副嗅球

Outline of Final Research Achievements

Olfaction is one of the most important perceptions for animals to receive information from their environments, such as in case of species preservation and danger avoidance. The information is transmitted through odor and pheromone substances, resulting in emotional, behavioral or physiological changes.
Here, we successfully observed odor responses that were evoked by odor and pheromone-like substances in the mouse whole brain, using functional MRI methods. Our developed analytical procedure efficiently identified mouse brain regions in which MR signal changes were synchronized with the periodic odor stimulations. Unique brain regions were activated by each odor and pheromone-like substance.

Research Products

• [Journal Article] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 3844-3844
  • Peer Reviewed
• [Journal Article] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 5363-5363
  • Peer Reviewed
• [Journal Article] Development of an awake mouse MRI method using soft immobilization for a cryogenic probe system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 1757-1757
  • Peer Reviewed
• [Journal Article] An iterative reconstruction method for dual-band EPI in small-animal studies. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 3242-3242
  • Peer Reviewed
• [Journal Article] Image-based phase correction for dual-band EPI with slice-GRAPPA using point-by-point procedures in k-space. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 4315-4315
  • Peer Reviewed
• [Journal Article] MEMRI and BOLD analyses of the olfactory perception system in response to odorant stimuli in mice. (2015)
  • Author(s): Funatsu, H., Yoshinaga, S., Goto, H., Hirakane, M., Iwamoto, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 23 Pages: 2033-2033
  • Peer Reviewed
• [Journal Article] Pre-scan with half-sized phase encoding blips reducing ghost and slice leakage artifacts in dual-band EPI. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 23 Pages: 2406-2406
  • Peer Reviewed
• [Journal Article] A novel reconstruction method for multi-band EPI in animal scanners with iterative point-by-point procedures in k-space. (2015)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Magn. Reson. Mat. Phys. Biol. Med. Volume: 28
  • Peer Reviewed
• [Presentation] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2017-04-22
  • Int'l Joint Research
• [Presentation] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2017-04-22
  • Int'l Joint Research
• [Presentation] 独立成分解析を利用したマウス嗅覚受容システムの BOLD 解析 (2017)
  • Author(s): 舩津大嗣、林芙優、吉永壮佐、杠直哉、草薙俊輔、武田光広、寺沢宏明
  • Organizer: ISMRM日本支部第１回学術集会
  • Place of Presentation: 脳情報通信融合研究センター、大阪
  • Year and Date: 2017-02-23
• [Presentation] Optimization of the awake mouse MRI method using mouse clothes for a cryogenic coil system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 第４４回日本磁気共鳴医学会大会
  • Place of Presentation: 大宮ソニックシティ、埼玉
  • Year and Date: 2016-09-10
• [Presentation] Detecting the olfactory responses in the mouse whole brain using a cryogenic probe. (2016)
  • Author(s): Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第４４回日本磁気共鳴医学会大会
  • Place of Presentation: 大宮ソニックシティ、埼玉
  • Year and Date: 2016-09-10
• [Presentation] fMRI analyses of the mouse olfactory perception system using a cryogenic probe. (2016)
  • Author(s): Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: The XXVIIth ICMRBS (International Conference on Magnetic Resonance in Biological Systems)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Development of an awake mouse MRI method using soft immobilization for a cryogenic probe system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] Image-based phase correction for dual-band EPI with slice-GRAPPA using point-by-point procedures in k-space. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] An iterative reconstruction method for dual-band EPI in small-animal studies. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] BOLD法を用いた匂い刺激によるマウス脳活性化の検出 (2015)
  • Author(s): 舩津大嗣、吉永壮佐、平金真、岩本成人、寺沢宏明
  • Organizer: 第５４回ＮＭＲ討論会
  • Place of Presentation: 千葉工業大学津田沼キャンパス、千葉
  • Year and Date: 2015-11-08
• [Presentation] A novel reconstruction method for multi-band EPI in animal scanners with iterative point-by-point procedures in k-space. (2015)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: ESMRMB (European Society for Magnetic Resonance in Medicine and Biology) 2015 32nd annual scientific meeting
  • Place of Presentation: Edinburgh International Conference Centre (EICC), Edinburgh, U.K.
  • Year and Date: 2015-10-01
  • Int'l Joint Research
• [Presentation] 極低温プローブに適用可能なマウス服を用いたawake MRI測定法の開発 (2015)
  • Author(s): 草薙俊輔、木村一成、平金真、岩本成人、荒木力太、吉永壮佐、寺沢宏明
  • Organizer: 第４３回日本磁気共鳴医学会大会
  • Place of Presentation: 東京ドームホテル、東京
  • Year and Date: 2015-09-11
• [Presentation] BOLD 法を用いた匂い刺激によるマウス嗅球の活性化の検出 (2015)
  • Author(s): 舩津大嗣、吉永壮佐、平金真、岩本成人、寺沢宏明
  • Organizer: 第４３回日本磁気共鳴医学会大会
  • Place of Presentation: 東京ドームホテル、東京
  • Year and Date: 2015-09-10
• [Presentation] Development of an awake mouse MR imaging method using soft immobilization for a cryogenic probe system. (2015)
  • Author(s): Kusanagi, S., Kimura, K., Funatsu, H., Hirakane, M., Iwamoto, S., Araki, R., Yoshinaga, S., and Terasawa, H.
  • Organizer: WMIC (World Molecular Imaging Congress) 2015
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] A robust Nyquist ghost suppression for high-resolution echo planar imaging of small animal brains. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Organizer: OHBM (Organization for Human Brain Mapping) annual conference 2015
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Presentation] MEMRI and BOLD analyses of the olfactory perception system in response to odorant stimuli in mice. (2015)
  • Author(s): Funatsu, H., Yoshinaga, S., Goto, H., Hirakane, M., Iwamoto, S., and Terasawa, H.
  • Organizer: 23rd ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] Pre-scan with half-sized phase encoding blips reducing ghost and slice leakage artifacts in dual-band EPI. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Organizer: 23rd ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] MRIを用いた匂い物質の識別メカニズムの解析 (2014)
  • Author(s): 後藤はるな、平金真、吉永壮佐、舩津大嗣、岩本成人、寺沢宏明
  • Organizer: 第４２回日本磁気共鳴医学会大会
  • Place of Presentation: ホテルグランヴィア京都（京都市）
  • Year and Date: 2014-09-19