| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Binding reaction analysis with cofactor in FMN-binding protein (FMN-bp) was performed. Binding strength with cofactor was thermodynamically analyzed by the surface plasmon resonance spectroscopy and isotherm titration calorimetry in addition to the fluorescence spectroscopy. The dissociation constant with cofactor was not affected by the phosphate ion concentration. By changing the preparation method of apoprotein, I could obtain a large amount of apoFMN-bp having binding activity, and determined the three-dimensional structure. These results were compared with other flavoproteins and new proposals were suggested on the binding mechanism of apoFMN-bp and FMN.
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