Cycloisomaltooligosaccharides from starch and their production and control mechanisms

• Project/Area Number: 26450133
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: FUNANE Kazumi 国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門 食品生物機能開発研究領域, 主席研究員 (90353953)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 環状イソマルトオリゴ糖 / 環状イソマルトオリゴ糖グルカノトランスフェラーゼ / 澱粉 / デキストラン / GH family 66 / デキストラナーゼ / αグルコシダーゼ / 不均化酵素 / GH family 31

Outline of Final Research Achievements

Cyclioisomaltooligosaccharide (CI)-producing bacteria, Paenibacillus sp. 598K produces CIs from starch with its two enzymes of cycloisomaltooligosaccharide glucanotransferase and dextranglucanase. The strain 598K release these two extracellular enzymes into the medium to produce CIs, intakes CIs, and then brakes them down with the intracellular dextranase, and finally use them as a source of nutrition. Another CI-producing bacterial strain Bacillus circulans T-3040 does not have dextranase to digest CIs. The strain T-3040 might not be able to utilize CIs, but it may be suitable for commercial CI-producing bacterial strain.

Research Products

• [Journal Article] Paenibacillus sp. 598K 6-α-glucosyltransferase is essential for cycloisomaltooligosaccharide synthesis from α-(1→4)-glucan (2017)
  • Author(s): Hitomi Ichinose, Ryuichiro Suzuki, Takatsugu Miyazaki, Keitarou Kimura, Mitsuru Momma, Nobuhiro Suzuki, Zui Fujimoto, Atsuo Kimura, Kazumi Funane
  • Journal Title: Applied Microbiology and Biotechnology Volume: 101 Issue: 10 Pages: 4115-4128
  • DOI: 10.1007/s00253-017-8174-z
  • NAID: 120006771683
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 環状イソマルトオリゴ糖はPaenibacillus sp. 598K由来GH family 66デキストラナーゼの生産に関与する (2017)
  • Author(s): 水島大貴, 宮崎剛亜, 木村啓太郎, 木村淳夫, 北村進一, 原博, 舟根和美
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
• [Presentation] Paenibacillus sp. 598K由来GH66デキストラナーゼの機能特性 (2016)
  • Author(s): 水島大貴，宮崎剛亜，志波優，吉川博文，木村啓太郎，木村淳夫，北村進一，原博，舟根和美
  • Organizer: 日本応用糖質科学会平成28年度大会
  • Place of Presentation: 福山大学(広島県福山市)
  • Year and Date: 2016-09-14
• [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼ生産菌における培地窒素源の酵素生産への影響 (2015)
  • Author(s): 宮崎剛亜, 木村啓太郎, 村上真, 鏡朋和, 舟根和美
  • Organizer: 日本応用糖質科学会平成27年度大会
  • Place of Presentation: 奈良春日野国際フォーラム （奈良県奈良市）
  • Year and Date: 2015-09-16