Regulatory mechanisms on an inductive production of flavonoid-type phytoalexin in rice

• Project/Area Number: 26450136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Okada Kazunori 東京大学, 生物生産工学研究センター, 准教授 (20312241)
• Co-Investigator(Renkei-kenkyūsha): HAMADA KOICHI 昭和薬科大学, 薬学系, 助教 (00343070)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 転写因子 / 二次代謝産物 / イネ / フラボノイド / ファイトアレキシン / ジャスモン酸 / OsMYC2 / OsMYL1 / OsMYL2 / 転写制御 / プロモーター

Outline of Final Research Achievements

Biosynthesis of sakuranetin, a flavonoid phytoalexin producedin rice, is highly dependent on jasmonic acid (JA) signalling. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT), is a key gene for sakuranetin production, but the regulation mechanism activating the sakuranetin production was unclear. This study showed that JA-inducible bHLH-type transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. Besides, we identified 2 interactors of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2 via their physical interaction. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice.

Research Products

• [Journal Article] OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability. (2017)
  • Author(s): Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, Okada K.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40175-40175
  • DOI: 10.1038/srep40175
  • Peer Reviewed / Open Access
• [Journal Article] OsMYC2 mediates numerous defence-related transcriptional changes via jasmonic acid signalling in rice. (2017)
  • Author(s): Ogawa S, Kawahara-Miki R, Miyamoto K, Yamane H, Nojiri H, Tsujii Y, Okada K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 486(3) Issue: 3 Pages: 796-803
  • DOI: 10.1016/j.bbrc.2017.03.125
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] イネのフラボノイド化合物サクラネチンの生産誘導におけるジャスモン酸と フォスファチジン酸の協調作用 (2017)
  • Author(s): 石田明大、小川哲史、西澤洋子、南栄一、山根久和、有村源一郎、野尻秀昭、岡田憲典
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学 (京都府 京都市)
  • Year and Date: 2017-03-17
• [Presentation] RERJ1, a JA-dependent Early Inducible bHLH Transcription Factor, Function in the rice JA-signaling System together with OsMYC2 and OsJAZ (2017)
  • Author(s): Ioana Valea, Koji Miyamoto, Kenji Gomi, Hideaki Nojiri, Kazunori Okada
  • Organizer: 日本植物整理学会
  • Place of Presentation: 鹿児島大学 (鹿児島県 鹿児島市)
  • Year and Date: 2017-03-16
• [Presentation] イネのジテルペン型ファイトアレキシン生産制御因子DPFの ジャスモン酸シグナル下流での働き (2016)
  • Author(s): 田渕 雄夢, 堤 涼, 根本 圭一郎, 澤崎 達也, 森 昌樹, 宮本 皓司, 山根 久和,光田 展隆,野尻 秀昭, 岡田 憲典
  • Organizer: 植物化学調節学会
  • Place of Presentation: 高知大学 (高知県 南国市)
  • Year and Date: 2016-10-28
• [Presentation] Rice JA inductive transcription factor RERJ1 regulates linalool emission in response to herbivory (2016)
  • Author(s): Motegi A, Miyamoto K, Yamane H, Shinya T, Galis I, Nojiri H, Okada K
  • Organizer: 14th International Symposium on Rice Functional Genomics
  • Place of Presentation: Le Corum (エロー県 モンペリエ)
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Regulation of diterpenoid phytoalexin biosynthesis mediated by the stress-inducible transcription factor DPF in rice (2016)
  • Author(s): Yumu Tabuchi, Ryo Tsutsumi, Keiichiro Nemoto, Tatsuya Sawasaki, Masaki Mori, Koji Miyamoto,Hisakazu Yamane, Nobutaka Mitsuda, Hideaki Nojiri, Kazunori Okada
  • Organizer: 2016 The International Plant Growth Substances Association meeting
  • Place of Presentation: University of Toronto (オンタリオ州 トロント）
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] イネの転写因子OsMYC2を介したサクラネチン生合成酵素遺伝子の転写制御機構 (2016)
  • Author(s): 小川哲史、宮本皓司、山根久和、野尻秀昭、岡田憲典
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] RERJ1, a Wound Inducible Transcription Factor - Involvement in the JA Signaling System (2016)
  • Author(s): Ioana Valea, Kenji Gomi, Hideaki Nojiri, Kazunori Okada
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] イネのフラボノイド型ファイトアレキシンであるサクラネチンの生物的ストレスに対する防御応答における役割 (2016)
  • Author(s): 石田明大、小川哲史、西澤洋子、南栄一、山根久和、有村源一郎、 野尻秀昭、岡田憲典
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] イネのサクラネチン生合成酵素遺伝子OsNOMTの転写を制御する因子の探索 (2015)
  • Author(s): 小川哲史、宮本皓司、山根久和、野尻秀昭、岡田憲典
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] イネのサクラネチン生合成酵素遺伝子を制御する転写因子の探索 (2015)
  • Author(s): 小川哲史、宮本皓司、山根久和、野尻秀昭、岡田憲典
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Transcriptional regulation of OsNOMT, the key gene in biosynthesis of a flavonoid phytoalexin in rice. (2014)
  • Author(s): Satoshi Ogawa, Koji Miyamoto, Hisakazu Yamane, Hideaki Nojiri, Kazunori Okada
  • Organizer: 第３回 日仏植物科学ワークショップ
  • Place of Presentation: 東京大学
  • Year and Date: 2014-10-27 – 2014-10-29
• [Presentation] イネのフラボノイド型ファイトアレキシン生合成酵素遺伝子の発現制御機構の解析 (2014)
  • Author(s): 小川 哲史, 宮本 皓司, 山根 久和, 野尻 秀昭, 岡田 憲典
  • Organizer: 植物化学調節学会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-18 – 2014-10-19
• [Presentation] Biological roles of sakuranetin, a flavonoid specialized metabolite inductively produced in rice. (2014)
  • Author(s): Satoshi Ogawa, Chie Yoshiga, Gen-ichiro Arimura, Takafumi Shimizu, Koji Miyamoto, Koichi Hamada, Yoko Nishizawa, Eiichi Minami, Hisakazu Yamane, Hideaki Nojiri, Kazunori Okada
  • Organizer: Plant Growth Regulation Society of America (PGRSA)
  • Place of Presentation: San Francisco, California, USA
  • Year and Date: 2014-06-13 – 2014-06-17