| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Bacteria communicate using chemical signals to sense cell density and regulate diverse coordinated behaviors by a process known as quorum sensing (QS). N-Acylhomoserine lactones (AHLs), one class of QS signals in Gram-negative bacteria, are synthesized from the acyl-acyl carrier protein (acyl-ACP) and
S-adenosylmethionine (SAM) by LuxI-type synthases. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM. This is the first study to show that QS signal synthases could be purified by a ligand-based affinity
protocol.
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