Identification of new biosynthetic and metabolic genes on strigolactones

• Project/Area Number: 26450144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Toyo University
• Principal Investigator: Umehara Mikihisa 東洋大学, 生命科学部, 教授 (30469895)
• Co-Investigator(Renkei-kenkyūsha): MIZUTANI Masaharu 神戸大学, 農学研究科, 准教授 (60303898)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ストリゴラクトン / 枝分かれ / 葉の老化 / LC-MS/MS / イネ / 代謝 / 硫黄欠乏 / D27 / 硫黄 / LC/MS-MS / シトクロムP450 / LC／MS－MS

Outline of Final Research Achievements

Strigolactones (SLs) are derived from beta-carotene. Beta-carotene isomerase, carotenoid cleavage dioxygenase (CCD) 7, CCD8, and cytochrome P450 are involved in SL biosynthesis. Plant roots can highly produce SLs in response to phosphorus and/or nitrogen deficiencies. In addition to the nutrient deficiencies, this study demonstrated that sulfur deficiency stimulated SL production in plant roots. The increased SL level is due to marked expression of D27 that encodes b-carotene isomerase.

Research Products

• [Journal Article] Possible roles of strigolactones during leaf senescence. (2015)
  • Author(s): Yusuke Yamada, Mikihisa Umehara
  • Journal Title: Plants Volume: 4 Issue: 3 Pages: 664-677
  • DOI: 10.3390/plants4030664
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural requirements of strigolactones for shoot branching inhibition in rice and Arabidopsis (2015)
  • Author(s): Mikihisa Umehara, Mengmeng Cao, Kohki Akiyama, Tomoki Akatsu, Yoshiya Seto, Atsushi Hanada, Li Weiqiang, Noriko Takeda-Kamiya, Yu Morimoto, Shinjiro Yamaguchi
  • Journal Title: Plant and cell physiology Volume: 印刷中 Issue: 6 Pages: 1059-1072
  • DOI: 10.1093/pcp/pcv028
  • Peer Reviewed
• [Journal Article] Strigolactone signaling regulates rice leaf senescence in response to a phosphate deficiency (2014)
  • Author(s): Yusuke Yamada, Soya Furusawa, Seiji Nagasaka, Koichiro Shimomura, Shinjiro Yamagucshi, Mikihisa Umehara
  • Journal Title: Planta Volume: 240 Issue: 2 Pages: 399-408
  • DOI: 10.1007/s00425-014-2096-0
  • Peer Reviewed
• [Presentation] 硫酸欠乏条件で栽培したイネのSL産生量 (2016)
  • Author(s): 進藤真登、中村華子、山田雄介、下村講一郎、山口信次郎、梅原三貴久
  • Organizer: 植物化学調節学会第51回大会
  • Place of Presentation: 高知大学農林海洋科学部（高知県南国市）
  • Year and Date: 2016-10-28
• [Presentation] ストリゴラクトンを介した植物の栄養応答 (2016)
  • Author(s): 梅原三貴久
  • Organizer: 第２回 植物の栄養研究会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-09-02
  • Invited
• [Presentation] 硫酸欠乏条件においてD27の高発現に伴ってストリゴラクトンが増加する (2016)
  • Author(s): 進藤真登、中村華子、山田雄介、下村講一郎、梅原三貴久
  • Organizer: 日本植物細胞分子生物学会 第34回大会
  • Place of Presentation: 信州大学繊維学部（長野県上田市）
  • Year and Date: 2016-09-01
• [Presentation] Sulfate deficiency stimulates strigolactone production in rice. (2016)
  • Author(s): Masato Shindo, Hanako Nakamura, Yusuke Yamada, Koichiro Shimomura, Mikihisa Umehara
  • Organizer: IPGSA conference 2016
  • Place of Presentation: トロント大学（カナダ・トロント）
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Remarks] 生命科学部 応用生物科学科 植物細胞工学研究室 Umehara Lab.
  • URL: http://www2.toyo.ac.jp/~umehara/plant_biotechnology/Welcome.html
• [Remarks] 生命科学部 応用生物科学科 植物細胞工学研究室 Umehara Lab.
  • URL: http://www2.toyo.ac.jp/~umehara/plant_biotechnology/Top.html