| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In this study, molecular design and synthesis of two transition-state analogue as inhibitors against hen-egg white lysozyme (HEWL). Furthermore, we investigated the catalytic mechanism of HEWL using these inhibitors. Next, it proved that the design principle of this inhibitor can also be applied to other polysaccharide-hydrolyzing enzyme. In addition, it was revealed that a part of the resulting oligosaccharide derivatives this time can be used as a substrate for enzyme activity measurement.
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