Molecular design and development of novel transition-state analogue based on reaction mechanism of hen-egg white lysozyme
Project/Area Number |
26450147
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bioorganic chemistry
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Research Institution | Fukushima National College of Technology |
Principal Investigator |
Ogata Makoto 福島工業高等専門学校, その他部局等, 准教授 (10532666)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | リゾチーム / 遷移状態アナログ / キチンオリゴ糖 / オリゴ糖 / 阻害剤 / 酵素 / 酵素阻害剤 / 多糖 / キチン |
Outline of Final Research Achievements |
In this study, molecular design and synthesis of two transition-state analogue as inhibitors against hen-egg white lysozyme (HEWL). Furthermore, we investigated the catalytic mechanism of HEWL using these inhibitors. Next, it proved that the design principle of this inhibitor can also be applied to other polysaccharide-hydrolyzing enzyme. In addition, it was revealed that a part of the resulting oligosaccharide derivatives this time can be used as a substrate for enzyme activity measurement.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] The structure of the proteinaceous inhibitor PliI from Aeromonas hydrophila in complex with its target lysozyme.2015
Author(s)
Leysen, S., Van Herreweghe, J. M., Yoneda, K., Ogata, M., Usui, T., Araki, T., Michiels, C. W., Strelkov, S. V.
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Journal Title
Acta Cryst.
Volume: D71
Issue: 2
Pages: 344-351
DOI
Related Report
Peer Reviewed
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[Journal Article] Non-catalytic dehydration of N,N’-diacetylchitobiose in high-temperature water.2014
Author(s)
Osada, M., Kikuta, K., Yoshida, K., Totani, K., Ogata, M., Usui, T.
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Journal Title
RSC Adv.
Volume: 4
Issue: 64
Pages: 33651-33657
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Interaction of di-N-acetylchitobiosyl moranoline with a family GH19 chitinase from moss, Bryum coronatum.2014
Author(s)
Shinya, S., Urasaki, A., Ohnuma, T., Taira, T., Suzuki, A., Ogata, M., Usui, T., Lampela, O., Juffer, AH., Fukamizo, T.
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Journal Title
Glycobiology
Volume: 24
Issue: 10
Pages: 945-955
DOI
Related Report
Peer Reviewed
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