| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The final goal of this project is to construct an efficient method for isolating new secondary metabolites from microbes and to isolate new compounds based on the methodology constructed here. For this purpose, following 4 tasks were carried out: (1) construction of a fraction library, (2) PDA-LC/MS analysis of the library, (3) construction of a spectral database named NPPlot, and (4) isolation of new metabolites from the library. As the results, a fraction library containing over 2,000 fractions was constructed from 30 L culture broths of several microbes. The library was analyzed by PDA-LC/MS for constructing the NPPlot. Several new compounds were isolated from the library by NPPlot screening, and the results were presented in conferences and published in academic journals. Through the project, I confirmed the efficiency of the method for isolating new metabolites from microbes.
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