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Studies of metabolic demands and de novo synthesis of dispensable amino acids

Research Project

Project/Area Number 26450157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

Ryuhei Kanamoto  京都大学, 農学研究科, 教授 (70147297)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords可欠アミノ酸 / セリン合成 / 3-ホスホグリセリン酸脱水素酵素 / タンパク質栄養 / タンパク質必要量 / 組織特異的発現 / ラット / 可決アミノ酸 / 3―ホスホグリセリン酸脱水素酵素
Outline of Final Research Achievements

Serine is a typical dispensable amino acid and known essentiality of its de novo synthesis. To clarify the metabolic demand of dispensable amino and its de novo synthesis, serine concentration and expression of PHGDH, the rate limiting enzyme of serine synthesis, was determined in several tissues of rat under different nutritional conditions and diabetes. The result shows that the protein nutrition most significantly affected the serine concentrations in tissues and plasm. It is found that serine concentration of liver, intestine and muscle was significantly decreased in diabetes rat. PHGDH was demonstrated to express ubiquitously in tissues investigated and the expression in liver, intestine and muscle was changed in response to protein nutrition. However, the clear relation between the expression of PHGDH and serine concentrations of tissues was not demonstrated.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(5 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (3 results)

  • [Journal Article] Down Regulation of Asparagine Synthetase and 3-Phosphoglycerate Dehydrogenase, and the Up-Regulation of Serine Dehydratase in Rat Liver from Intake of Excess Amount of Leucine Are Not Related to Leucine-Caused Amino Acid Imbalance2015

    • Author(s)
      Yoshimura, R., Takai, M., Namaki, H., Minami, K., Imamura, W., Kato, H., Kamei, Y., and Kanamoto, R.
    • Journal Title

      J Nutr Sci Vitaminol

      Volume: 61 Issue: 6 Pages: 441-448

    • DOI

      10.3177/jnsv.61.441

    • NAID

      130005125480

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] タンパク質栄養の認識とアスパラギン合成酵素(AS)と3-ホスホグリセリン酸脱水素酵素(PHGDH)の発現制御2014

    • Author(s)
      金本龍平、二川みどり、吉村亮二、亀井康富
    • Journal Title

      アミノ酸研究

      Volume: 8 Pages: 155-156

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 低糖質食がアミノ酸代謝に及ぼす影響 -各種臓器におけるアミノ酸濃度の変化とAS,PHGDH及び肝臓SDHの発現-2017

    • Author(s)
      Shim Yeshin、 大日向耕作、金本龍平
    • Organizer
      第71回日本栄養・食糧学会大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2017-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 糖尿病によるラット各種臓器における可欠アミノ酸代謝酵素(AS,PHGDH,SDH)の発現変動2016

    • Author(s)
      沈 礼信、大日向耕作、金本龍平
    • Organizer
      第70回 日本栄養・食糧学会大会
    • Place of Presentation
      武庫川女子大学中央キャンパス
    • Year and Date
      2016-05-13
    • Related Report
      2015 Research-status Report
  • [Presentation] タンパク質栄養の認識とアスパラギン合成酵素(AS)と3-ホスホグリセリン酸脱水素酵素(PHGDH)の発現制御2014

    • Author(s)
      金本龍平、二川みどり、吉村亮二、亀井康富
    • Organizer
      日本アミノ酸学会第8回学術大会
    • Place of Presentation
      東京農業大学世田谷キャンパス
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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