Studies of metabolic demands and de novo synthesis of dispensable amino acids

• Project/Area Number: 26450157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: Ryuhei Kanamoto 京都大学, 農学研究科, 教授 (70147297)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 可欠アミノ酸 / セリン合成 / 3-ホスホグリセリン酸脱水素酵素 / タンパク質栄養 / タンパク質必要量 / 組織特異的発現 / ラット / 可決アミノ酸 / 3―ホスホグリセリン酸脱水素酵素

Outline of Final Research Achievements

Serine is a typical dispensable amino acid and known essentiality of its de novo synthesis. To clarify the metabolic demand of dispensable amino and its de novo synthesis, serine concentration and expression of PHGDH, the rate limiting enzyme of serine synthesis, was determined in several tissues of rat under different nutritional conditions and diabetes. The result shows that the protein nutrition most significantly affected the serine concentrations in tissues and plasm. It is found that serine concentration of liver, intestine and muscle was significantly decreased in diabetes rat. PHGDH was demonstrated to express ubiquitously in tissues investigated and the expression in liver, intestine and muscle was changed in response to protein nutrition. However, the clear relation between the expression of PHGDH and serine concentrations of tissues was not demonstrated.

Research Products

• [Journal Article] Down Regulation of Asparagine Synthetase and 3-Phosphoglycerate Dehydrogenase, and the Up-Regulation of Serine Dehydratase in Rat Liver from Intake of Excess Amount of Leucine Are Not Related to Leucine-Caused Amino Acid Imbalance (2015)
  • Author(s): Yoshimura, R., Takai, M., Namaki, H., Minami, K., Imamura, W., Kato, H., Kamei, Y., and Kanamoto, R.
  • Journal Title: J Nutr Sci Vitaminol Volume: 61 Issue: 6 Pages: 441-448
  • DOI: 10.3177/jnsv.61.441
  • NAID: 130005125480
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] タンパク質栄養の認識とアスパラギン合成酵素(AS)と３－ホスホグリセリン酸脱水素酵素(PHGDH)の発現制御 (2014)
  • Author(s): 金本龍平、二川みどり、吉村亮二、亀井康富
  • Journal Title: アミノ酸研究 Volume: 8 Pages: 155-156
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 低糖質食がアミノ酸代謝に及ぼす影響 －各種臓器におけるアミノ酸濃度の変化とAS,PHGDH及び肝臓SDHの発現－ (2017)
  • Author(s): Shim Yeshin、 大日向耕作、金本龍平
  • Organizer: 第７１回日本栄養・食糧学会大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2017-05-19
• [Presentation] 糖尿病によるラット各種臓器における可欠アミノ酸代謝酵素（AS,PHGDH,SDH）の発現変動 (2016)
  • Author(s): 沈 礼信、大日向耕作、金本龍平
  • Organizer: 第７０回 日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学中央キャンパス
  • Year and Date: 2016-05-13
• [Presentation] タンパク質栄養の認識とアスパラギン合成酵素(AS)と３－ホスホグリセリン酸脱水素酵素(PHGDH)の発現制御 (2014)
  • Author(s): 金本龍平、二川みどり、吉村亮二、亀井康富
  • Organizer: 日本アミノ酸学会第８回学術大会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2014-11-08 – 2014-11-09