Flow-injectin ESR study for superoxide radical scavenging activity of antioxidants of vegetables
| Project/Area Number |
26450158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Tajima Kunihiko 京都工芸繊維大学, 分子化学系, 教授 (50163457)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | スーパーオキシドラジカル / アルコキシルラジカル / 抗酸化活性 / 青果物 / 流通型ESR装置 / 活性酸素 / スピントラッピングESR / HPLC-ESR装置 / コーヒー酸誘導体 / 不飽和脂肪酸 / トマト / ほうれん草 / 青果物の抗酸化活性 / HPLC-ESR装置開発 |
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| Outline of Final Research Achievements |
A new flow-injection ESR (FI-ESR) systems special for quantitative spin-trapping ESR detection of alkoxyl (RO) radicals were developed to evaluate the alkoxy radical elimination capacity (AREC) of plants derived potent antioxidants. The AREC system was enable to observe the DMPO (5,5-dimethyl-1-pyrroline N-oxide) spin adducts of the alkoxyl radical produced by thermal decomposition of AAPH (2,2'-azobis(2,4-amidinopropane) dihydrochloride) using the flow-injection ESR (FI-ESR) method. The AREC value of plants derived phenolic compounds, such as caffeic acid, catechine, and rutine, were determined by using Trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid, TRX) as a standard sample. The newly defined AREC value is applicable for various biosubstances, and is far more excellent and reliable indicator than the oxygen radical absorption capacity (ORAC) value determined by the well-known ORAC-fluorescein assay methods.
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Report
(4 results)
Research Products
(22 results)
