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Flow-injectin ESR study for superoxide radical scavenging activity of antioxidants of vegetables

Research Project

Project/Area Number 26450158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionKyoto Institute of Technology

Principal Investigator

Tajima Kunihiko  京都工芸繊維大学, 分子化学系, 教授 (50163457)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsスーパーオキシドラジカル / アルコキシルラジカル / 抗酸化活性 / 青果物 / 流通型ESR装置 / 活性酸素 / スピントラッピングESR / HPLC-ESR装置 / コーヒー酸誘導体 / 不飽和脂肪酸 / トマト / ほうれん草 / 青果物の抗酸化活性 / HPLC-ESR装置開発
Outline of Final Research Achievements

A new flow-injection ESR (FI-ESR) systems special for quantitative spin-trapping ESR detection of alkoxyl (RO) radicals were developed to evaluate the alkoxy radical elimination capacity (AREC) of plants derived potent antioxidants. The AREC system was enable to observe the DMPO (5,5-dimethyl-1-pyrroline N-oxide) spin adducts of the alkoxyl radical produced by thermal decomposition of AAPH (2,2'-azobis(2,4-amidinopropane) dihydrochloride) using the flow-injection ESR (FI-ESR) method. The AREC value of plants derived phenolic compounds, such as caffeic acid, catechine, and rutine, were determined by using Trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid, TRX) as a standard sample. The newly defined AREC value is applicable for various biosubstances, and is far more excellent and reliable indicator than the oxygen radical absorption capacity (ORAC) value determined by the well-known ORAC-fluorescein assay methods.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) Book (1 results)

  • [Journal Article] Molecular Structure of Poly(vinyl alcohol)-Derived Carbon-Centered Radicals Studied by Rapid-Flow and Spin-Trapping ESR Measurements: A Short-Lived Intermediate Radical in the Initial Stage of the Graft Polymerization Reaction2017

    • Author(s)
      Yuko Nishihara, Kazuma Matsui, Keiko Kuwabara, Naoya Murata, Tomoko Yamaguchi, Yusuke Miyake, Tohru Ikegami, Kenji Kanaori, and Kunihiko Tajima
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 90 Issue: 2 Pages: 244-253

    • DOI

      10.1246/bcsj.20160332

    • NAID

      130005332366

    • ISSN
      0009-2673, 1348-0634
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Accuracy and Validity of AREC (Alkoxy Radical Elimination Capacity) Assay in Evaluating the Antioxidant Abilities of Various Biosubstances2017

    • Author(s)
      Akira Nakajima, Tomoko Yamaguchi, Gen Hattori, Yasuhiro Sakurai, Masaki Kawamura, Kiyoshi Kawai, Yusuke Miyake, Kenji Kanaori, Kunihiko Tajima
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 90 Issue: 2 Pages: 223-230

    • DOI

      10.1246/bcsj.20160355

    • NAID

      130005332360

    • ISSN
      0009-2673, 1348-0634
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of iron-sulfur clusters in flavin-containing opine dehydrogenase2016

    • Author(s)
      S. Watanabe, Kunihiko. Tajima, K. Matsui, Y. Watanabe
    • Journal Title

      Bioscience, Biotech. Biochem.,

      Volume: 80 Issue: 12 Pages: 2371-2375

    • DOI

      10.1080/09168451.2016.1206812

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase2016

    • Author(s)
      Seiya Watanabe, Kunihiko Tajima, Satoshi Fujii, Fumiyasu Fukumori, Ryotaro Hara, Rio Fukuda, Mao Miyazaki, Kuniki Kino & Yasuo Watanabe
    • Journal Title

      Scientific Reports

      Volume: 08 Issue: 1 Pages: 38720-38720

    • DOI

      10.1038/srep38720

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reactive oxygen species inhibitory diagrams and their usability for the evaluation of antioxidant ability2016

    • Author(s)
      Akira Nakajima, Yasuhiro Sakurai, Kunihiko Tajima
    • Journal Title

      Oxide Antioxid Med Sci.

      Volume: 5 Pages: 1-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of a Flow-injection Spin-trapping ESR Method for Evaluating the Alkoxy Radical Elimination Capacity (AREC) of Selected Antioxidants2015

    • Author(s)
      Yasuhiro Ssakurai, Tomoko Yamaguchi, Akira Nakajima, Kenji Kanaori, and Kunihiko Tajima
    • Journal Title

      Chemistry Letters

      Volume: 44 Issue: 6 Pages: 752-754

    • DOI

      10.1246/cl.150121

    • NAID

      130005074599

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay2015

    • Author(s)
      Akira Nakajima,Tomoko Yamaguchi,Tomoyuki Yamashita,Kiyoshi Kawai,Yusuke Miyake,Kenji Kanaori,Kunihiko Tajima
    • Journal Title

      Applied Magnetic Resonance

      Volume: 46 Issue: 9 Pages: 1-10

    • DOI

      10.1007/s00723-015-0664-5

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 流通型ESR法による脂溶性抗酸化物質のアルコキシルラジカル消去活性評価2017

    • Author(s)
      山口 智子、中島 暉、三宅 祐輔、金折 賢二、田嶋 邦彦
    • Organizer
      日本化学会春期年会
    • Place of Presentation
      慶応大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] ストップトフロー分光法によるフラボノイド由来セミキノンラジカルの生成消失機構解析2017

    • Author(s)
      桑原 慶子、山口 智子、三宅 祐輔、金折 賢二、田嶋 邦彦
    • Organizer
      日本化学会春期年会
    • Place of Presentation
      慶応大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] HPLC-ESR 法による米酢および紅芋酢に含まれる O2-消去活性物質の評価2016

    • Author(s)
      山口智子,川村雅樹,三宅祐輔,金折賢二,飯尾 毅,田嶋邦彦
    • Organizer
      第63回食品科学工学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] HPLC-ESR 法によるトマトに含まれる水溶性成分の O2- 消去活性評価2016

    • Author(s)
      櫻井康博,山口智子,服部 玄,有井雅幸,三宅祐輔,金折賢二,田嶋邦彦
    • Organizer
      第63回食品科学工学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] HPLC-ESR法による青果物に含まれる水溶性成分のO2-・消去活性物質の探索2015

    • Author(s)
      山口 智子・服部 玄・三宅 祐輔・金折 賢二・田嶋 邦彦
    • Organizer
      第54回電子スピンサイエンス学会
    • Place of Presentation
      新潟市朱鷺メッセ
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
  • [Presentation] HPLC-ESR法によるほうれん草に含まれる水溶性成分のO2-・消去活性評価2015

    • Author(s)
      山口智子・服部玄・三宅祐輔・金折賢二・中島暉 ・田嶋邦彦
    • Organizer
      第62回日本食品科学工学会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Presentation] HPLC-ESR法による青果物に含まれる水溶性成分のO2-・消去活性物質の探索2015

    • Author(s)
      山口 智子・服部 玄・三宅 祐輔・金折 賢二・田嶋 邦彦
    • Organizer
      第62回日本食品科学工学会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Presentation] HPLC-ESR法によるカラム溶出成分のO2-・消去活性評価方法,2015

    • Author(s)
      山口 智子・服部 玄・ 三宅 祐輔・金折 賢二・田嶋 邦彦
    • Organizer
      第19回ESRフォーラム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 抗酸化物質のアルコキシラジカル消去能評価への流通型スピントラッピングESR法の応用2015

    • Author(s)
      中島 暉・山下 智之・山口智子・川井 清史・三宅祐輔・金折賢二・田嶋邦彦
    • Organizer
      第19回ESRフォーラム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
  • [Presentation] 流通型ESR法による生体関連フェノール誘導体とO2-・の二次反応速度の解析2015

    • Author(s)
      山下 智之・櫻井 康博・金折 賢二・田嶋 邦彦
    • Organizer
      第19回ESRフォーラム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
  • [Presentation] HPLC-ESR法によるカラム溶出成分のO2-・消去活性評価方法2015

    • Author(s)
      山口智子, 田中宏治, 三宅祐輔, 金折賢二, 田嶋邦彦
    • Organizer
      日本化学会 第95春季年会
    • Place of Presentation
      日本大学 理工学部船橋キャンパス
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Superoxide radical scavenging activity of cooked rice as studied by HPLC-ESR spin-trapping measurements2014

    • Author(s)
      Tomoko Yamaguchi , Gen Httori, Kenji Kanaori, Kunihiko Tajima, Isamu Tachimoto
    • Organizer
      APES-IES-SEST 2014
    • Place of Presentation
      東大寺文化センター
    • Year and Date
      2014-11-12 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] HPLC-ESR法による炊飯米の水溶性成分のO2-・消去活性評価2014

    • Author(s)
      山口 智子, 服部 玄, 金折 賢二, 田嶋 邦彦, 舘本 勲武
    • Organizer
      61回日本食品科学工学会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] HPLC-ESR法による炊飯米水溶性成分のO2-・消去活性評価2014

    • Author(s)
      山口 智子, 服部 玄, 金折 賢二, 田嶋 邦彦, 舘本 勲武
    • Organizer
      第18回ESRフォーラム
    • Place of Presentation
      名古屋大学シンポジオン会議室
    • Year and Date
      2014-07-26
    • Related Report
      2014 Research-status Report
  • [Book] 活性酸素・フリーラジカルの科学2016

    • Author(s)
      日本化学会 編
    • Total Pages
      181
    • Publisher
      化学同人
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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