Study on the dietary components which affect incretin secresion by regulating the function of plasma membrane in enteroendocrine cells

• Project/Area Number: 26450162
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Saga University
• Principal Investigator: Mitsutake Susumu 佐賀大学, 農学部, 准教授 (10344475)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: GPR120 / インクレチン

Outline of Final Research Achievements

In this study, we examined how the sphingolipids, polyphenols, or polysaccharide in food affect the function of plasma membrane on enteroendocrine cells, We focused on the receptor of long-chain fatty acid, GPR120, and developed a new method to assay fatty acid/ GPR120 signaling employing TGF alpha- shedding assay. The method was convenient and showed high quantity. Using this method, we found several new ligand and molecules which affect alpha linolenic acid/ GPR120 signaling.

Research Products

• [Journal Article] Possible roles of long-chain sphingomyelines and sphingomyelin synthase 2 in mouse macrophage inflammatory response. (2017)
  • Author(s): Sakamoto H, Yoshida T, Sanaki T, Shigaki S, Morita H, Oyama M, Mitsui M, Tanaka Y, Nakano T, Mitsutake S, Igarashi Y, Takemoto H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 482 Issue: 2 Pages: 202-207
  • DOI: 10.1016/j.bbrc.2016.11.041
  • Peer Reviewed
• [Journal Article] 細胞膜スフィンゴミエリンの代謝制御による新規細胞膜ダイナミズム (2017)
  • Author(s): 光武 進
  • Journal Title: 生化学 Volume: 89 Pages: 86-89
  • NAID: 40021119790
• [Journal Article] Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus (2016)
  • Author(s): M Taniguchi, T Tasaki, H Ninomiya, Y Ueda, K Kuremoto, S Mitsutake, Y Igarashi, T Okazaki, T Takegami
  • Journal Title: Scientific Reports Volume: 6 Issue: 1
  • DOI: 10.1038/srep37829
  • Peer Reviewed / Open Access
• [Journal Article] Chemical analysis of the sugar moiety of monohexosylceramide contained in koji, Japanese traditional rice fermented with Aspergillus. (2016)
  • Author(s): Hiroshi Hamajima, Ayumi Fujikawa, Mikako Yamashiro, Takatoshi Ogami, Seiichi Kitamura, Masahito Tsubata, Sei Tan, Haruka Matsunaga, Kazutaka Sawada, Satoshi Kumagai, Nobuyuki Hayashi, Koji Nagao, Teruyoshi Yanagita, Takuji Oka, Susumu Mitsutake and Hiroshi Kitagaki*
  • Journal Title: Fermentation Volume: 2 Issue: 1 Pages: 2-2
  • DOI: 10.3390/fermentation2010002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Glucosylceramide contained in Koji mold-cultured cereal confers membrane and flavor modification and stress tolerance to Saccharomyces cerevisiae during coculture fermentation. (2015)
  • Author(s): Kazutaka Sawada, Tomoya Sato, Hiroshi Hamajima, Lahiru Niroshan Jayakody, Miyo Hirata, Mikako Yamashiro, Marie Tajima, Susumu Mitsutake, Koji Nagao, Keisuke Tsuge, Fumiyoshi Abe, Kentaro Hanada, and Hiroshi Kitagaki*
  • Journal Title: Applied and Environmental Microbiology, 81(11):3688-98. Volume: 81 Issue: 11 Pages: 3688-3698
  • DOI: 10.1128/aem.00454-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sphingomyelin Synthase 2, but Not Sphingomyelin Synthase 1, Is Involved in HIV-1 Envelope-mediated Membrane Fusion. (2014)
  • Author(s): Yasuhiro Hayashi, Yoko Nemoto-Sasaki, Takashi Tanikawa, Saori Oka, Kiyoto Tsuchiya, Kouta Zama, Susumu Mitsutake, Takayuki Sugiura and Atsushi Yamashita
  • Journal Title: J Biol Chem. Volume: 289 Issue: 44 Pages: 30842-30856
  • DOI: 10.1074/jbc.m114.574285
  • Peer Reviewed
• [Journal Article] Decreased amyloid-beta pathologies by intracerebral loading of glycosphingolipid-enriched exosomes in Alzheimer model mice. (2014)
  • Author(s): Yuyama, K., Sun, H., Sakai, S., Mitsutake, S., Okada, M., Tahara, H., Furukawa, J., Fujitani, N., Shinohara, Y. & Igarashi, Y.
  • Journal Title: J Biol Chem Volume: 289 Issue: 35 Pages: 24488-24498
  • DOI: 10.1074/jbc.m114.577213
  • Peer Reviewed / Open Access
• [Journal Article] Altered levels of serum sphingomyelin and ceramide containing distinct acyl chains in young obese adults. (2014)
  • Author(s): H. Hanamatsu, S. Ohnishi, S. Sakai, K. Yuyama, S. Mitsutake, H. Takeda, Y. Igarashi
  • Journal Title: Nutrition & Diabetes Volume: 4 Issue: 10 Pages: e141-e141
  • DOI: 10.1038/nutd.2014.38
  • Peer Reviewed
• [Journal Article] Pathological roles of ceramide and its metabolites in metabolic syndrome and Alzheimer's disease (2014)
  • Author(s): K. Yuyama, S. Mitsutake, Y. Igarashi
  • Journal Title: Biochimica et Biophysica Acta -Molecular and Cell Biology of Lipids Volume: 1841 Issue: 5 Pages: 793-798
  • DOI: 10.1016/j.bbalip.2013.08.002
  • Peer Reviewed
• [Presentation] 「細胞膜脂質と食品機能」 (2016)
  • Author(s): 光武進
  • Organizer: 日本農芸化学会 西日本支部大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2016-09-15
  • Invited
• [Presentation] 脂肪酸受容体GPR120に対するポルフィランオリゴ糖の作用 (2015)
  • Author(s): 中道ひかる、大串麻衣、濱洋一郎、光武進
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] スフィンゴミエリンのインスリン抵抗性／NAFLDへの関与 (2014)
  • Author(s): 光武進、花松久寿、大西俊介、酒井翔太、五十嵐靖之
  • Organizer: 日本農芸化学会西日本支部大会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2014-09-18 – 2014-09-19
• [Presentation] スフィンゴ脂質合成／代謝酵素による細胞膜機能制御 (2014)
  • Author(s): 光武進
  • Organizer: スフィンゴ脂質合成／代謝酵素による細胞膜機能制御
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] スフィンゴ脂質合成／代謝制御による細胞膜機能制御とその破綻によるメタボリックシンドローム (2014)
  • Author(s): 光武進
  • Organizer: 第８回 レドックス・ライフイノベーション第170委員会
  • Place of Presentation: 宮崎
  • Year and Date: 2014-08-21 – 2014-08-23
  • Invited