Induction of brown-like adipocytes in white adipose tissue by food-derived factors

• Project/Area Number: 26450168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Chubu University
• Principal Investigator: TSUDA Takanori 中部大学, 応用生物学部, 教授 (90281568)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 食品由来因子 / 肥満

Outline of Final Research Achievements

Induction of brown-like adipocytes in white adipose tissue suggests
a new approach for preventing and treating obesity via induction of thermogenesis aasociated with uncoupling protein 1. Here, we demonstrate that several food-derived factors significantly induces brown-like adipocytes in cells and mice. These findings may provide insight into browning of white adipocytes including the molecular mechanism mediated by food-derived factors and demonstrate that food-derived factors have a novel biological function with regard to increasing energy expenditure by browning of white adipocytes.

Research Products

• [Journal Article] Curcumin improves glucose tolerance via stimulation of glucagon-like peptide-1 secretion. (2017)
  • Author(s): Kato, M., Nishikawa, S., Ikehata, A., Dochi, K., Tani, T., Takahashi, T., Imaizumi, A., Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res. Volume: 61 Issue: 3 Pages: 1600471-1600471
  • DOI: 10.1002/mnfr.201600471
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low molecular fraction of wheat protein hydrolysate stimulates glucagon-like peptide-1 secretion in an enteroendocrine L cell line and improves glucose tolerance in rats. (2017)
  • Author(s): Kato, M., Nakanishi, T., Tani., T., Tsuda, T.
  • Journal Title: Nutr. Res. Volume: 37 Pages: 37-45
  • DOI: 10.1016/j.nutres.2016.12.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recent progress in anti-obesity and anti-diabetes effect of berries. (2016)
  • Author(s): Tsuda, T.
  • Journal Title: Antioxidants Volume: 5 Issue: 2 Pages: 13-13
  • DOI: 10.3390/antiox5020013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Artepillin C, a typical Brazilian propolis-derived component, induces brown-like adipocyte formation in C3H10Ｔ1/2 cells, primary inguinal white adipose tissue-derived adipocytes, and mice. (2016)
  • Author(s): Nishikawa, S., Aoyama, H., Kamiya, M., Higuchi, J., Kato, A., Soga, M., Kawai, T., Yoshimura, K., Kumazawa, S., Tsuda, T.
  • Journal Title: PLoS ONE Volume: 11 Issue: 9 Pages: e0162512-e0162512
  • DOI: 10.1371/journal.pone.0162512
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 食用色素アントシアニンのサイエンス－化学、体内動態と機能、将来展望－ (2016)
  • Author(s): 津田孝範
  • Journal Title: ニューフードインダストリ― Volume: 58 Pages: 11-20
  • NAID: 40020780696
• [Journal Article] Possible abilities of dietary factors to prevent and treat diabetes via the stimulation of glucagon-like peptide-1 secretion. (2015)
  • Author(s): Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res. Volume: 59 Issue: 7 Pages: 1264-1273
  • DOI: 10.1002/mnfr.201400871
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The anthocyanin delphinidin 3-rutinoside stimulates glucagon-like peptide-1 secretion in murine GLUTag Cell Line via the Ca2+/Calmodulin-dependent kinase II Pathway. (2015)
  • Author(s): Kato, M., Tani, T., Tsuda, T.
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: e0126157-e0126157
  • DOI: 10.1371/journal.pone.0126157
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1). (2014)
  • Author(s): Nagamine, R., Ueno, S., Tsubata, M., Yamaguchi, K., Takagaki, K., Hira, T., Hara, H., Tsuda, T.
  • Journal Title: Food & Function Volume: 5 Issue: 9 Pages: 2309-2316
  • DOI: 10.1039/c4fo00032c
  • Peer Reviewed
• [Journal Article] The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. (2014)
  • Author(s): Soga, M., Ohashi, A., Taniguchi, M., Matsui, T., Tsuda, T.
  • Journal Title: FEBS Open Bio Volume: 4 Issue: 1 Pages: 898-904
  • DOI: 10.1016/j.fob.2014.10.008
  • Peer Reviewed / Open Access
• [Journal Article] 特集 食品と疾病 ２．アントシアニンアントシアニンのADME（吸収・分布・代謝・排泄） (2014)
  • Author(s): 津田孝範
  • Journal Title: Functional Food Volume: 8 Pages: 74-78
• [Journal Article] 特集 食品と疾病 ６．アントシアニンとメタボリックシンドローム (2014)
  • Author(s): 津田孝範
  • Journal Title: Functional Food Volume: 8 Pages: 99-102
• [Presentation] クルクミンによる褐色脂肪細胞化の誘導とその機序解明 (2017)
  • Author(s): 西川 翔、神谷 美沙、青山 広樹、野村 真美、高橋 司、今泉 厚、津田 孝範
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学（京都府京都市）
  • Year and Date: 2017-03-17
• [Presentation] Possible role of anthocyanins in the prevention and treatment of diabetes via the stimulation of GLP-1 secretion. (2017)
  • Author(s): Tsuda, T.
  • Organizer: 9th International Workshop on Anthocyanins
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2017-02-22
  • Int'l Joint Research
• [Presentation] プロポリス成分による褐色脂肪細胞化誘導と機構解明 (2017)
  • Author(s): 青山広樹、西川 翔、神谷美沙、吉村一輝、熊澤茂則、津田孝範
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学（京都府京都市）
• [Presentation] プロポリス成分による褐色脂肪細胞化誘導と機構解明 (2016)
  • Author(s): 青山広樹、西川 翔、神谷美沙、吉村一輝、熊澤茂則、津田孝範
  • Organizer: 第71回日本栄養・食糧学会中部支部大会
  • Place of Presentation: 岐阜大学（岐阜県岐阜市）
  • Year and Date: 2016-11-19
• [Presentation] 鮮やかな色素、アントシアニンの健康機能－スーパーフルーツ“ワイルドブルーベリー”の活用－ (2016)
  • Author(s): 津田孝範
  • Organizer: 北米ワイルドブルーベリーシンポジウム
  • Place of Presentation: 駐日カナダ大使館（東京都港区）
  • Year and Date: 2016-10-05
  • Invited
• [Presentation] 鮮やかな植物色素、アントシアニンの健康機能－研究動向と課題－ (2016)
  • Author(s): 津田孝範
  • Organizer: 園芸学会平成28年度秋季大会公開シンポジウム
  • Place of Presentation: 名城大学（愛知県名古屋市）
  • Year and Date: 2016-09-10
  • Invited
• [Presentation] プロポリスおよびローヤルゼリー構成成分の健康機能開拓 (2016)
  • Author(s): 西川翔、津田孝範
  • Organizer: 日本食品科学工学会第63回大会シンポジウム
  • Place of Presentation: 名城大学（愛知県名古屋市）
  • Year and Date: 2016-08-25
  • Invited
• [Presentation] 消化管ホルモン分泌とクルクミン、糖尿病予防 (2016)
  • Author(s): 津田孝範
  • Organizer: 日本食品科学工学会第63回大会シンポジウム
  • Place of Presentation: 名城大学（愛知県名古屋市）
  • Year and Date: 2016-08-25
  • Invited
• [Presentation] 食品由来因子による褐色脂肪細胞化誘導と機構解明 (2016)
  • Author(s): 西川 翔、青山 広樹、神谷 美沙、樋口 潤、加藤 愛子、河合 妙子、曽我 実、津田 孝範
  • Organizer: 第70回 日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-13
• [Presentation] クルクミンによるGLP-1分泌促進作用と血糖値制御 (2016)
  • Author(s): 加藤正樹、谷翼、津田孝範
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-27
• [Presentation] 褐色脂肪細胞化を誘導する食品由来因子とその機序解明 (2016)
  • Author(s): 西川翔、青山広樹、神谷美沙、樋口潤、加藤愛子、河合妙子、曽我実、津田孝範
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-27
• [Presentation] 消化管ホルモン分泌から考えるクルクミンの作用と糖尿病予防 (2016)
  • Author(s): 津田孝範
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-09
  • Invited
• [Presentation] Possibility of dietary factors to prevent and treat diabetes via the stimulation of GLP-1 secretion. (2015)
  • Author(s): Tsuda, T.
  • Organizer: Pacifichem2015
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] アントシアニンによるGLP-1の分泌促進作用とその分泌経路の解明 (2015)
  • Author(s): 谷翼、加藤正樹、津田孝範
  • Organizer: 第61回日本食品科学工学会大会
  • Place of Presentation: 京都大学（京都市）
  • Year and Date: 2015-08-27
• [Presentation] The anthocyanin delphinidin 3-rutinoside stimulates GLP-1 secretion (2015)
  • Author(s): Tani, T., Kato, M., Tsuda, T.
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: Pacifico Yokohama (Yokohama)
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] Wheat protein hydrolysate stimulates GLP-1 secretion in murine enteroendocrine L cell model and rats. (2015)
  • Author(s): Kato, M., Nakanishi, T., Tani, T., Tsuda, T.
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: Pacifico Yokohama (Yokohama)
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] アントシアニンによるGLP-1分泌促進作用とその分泌経路の解明 (2015)
  • Author(s): 谷翼、加藤正樹、津田孝範
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県・岡山市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 小麦タンパク質加水分解物によるGLP-1分泌促進機構の解明 (2015)
  • Author(s): 加藤正樹、中西健悟、谷翼、津田孝範
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県・岡山市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] GLP-1の分泌を促進する食品因子とその分泌経路の解明 (2014)
  • Author(s): 谷翼、加藤正樹、津田孝範
  • Organizer: 第67回 日本栄養・食糧学会中部支部大会
  • Place of Presentation: 静岡県立大学（静岡県・静岡市）
  • Year and Date: 2014-11-22
• [Presentation] Prevention of Diabetes Using Berry Anthocyanins Via Activation of AMPK and Stimulation of GLP-1 Secretion. (2014)
  • Author(s): Tsuda, T.
  • Organizer: WBANA 17th Health Research Summit
  • Place of Presentation: Bar Harbor, ME, USA
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] Curcumin stimulates glucagon-like peptide-1 secretion via Ca2+/calmodulin-dependent kinase II activation and ameliorates glucose intolerance. (2014)
  • Author(s): Kato, M., Tani, T., Tsuda, T.
  • Organizer: 27th International Conference on Polyphenols
  • Place of Presentation: Nagoya University, Nagoya, Aichi
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] Sweet potato (Ipomoea batatas L.) leaf extract increases glucagon-like peptide-1 (GLP-1) release and attenuates hyperglycemia in rodent models. (2014)
  • Author(s): Nagamine, R., Ueno, S., Tsubata, M., Yamaguchi, K., Takagaki, K., Hira, T., Hara, H., Tsuda, T.
  • Organizer: 27th International Conference on Polyphenols
  • Place of Presentation: Nagoya University, Nagoya, Aichi
  • Year and Date: 2014-09-02 – 2014-09-06
• [Presentation] 血管弛緩性ペプチド Trp-His（WH）によるAMP キナーゼ活性化作用と機序解明 (2014)
  • Author(s): 曽我実、大橋彩加、谷口恵実、滝川雅仁、松井利郎、津田孝範
  • Organizer: 日本食品科学工学会第61回大会
  • Place of Presentation: 中村学園大学（福岡県・福岡市）
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] クルクミンと消化管ホルモン分泌 (2014)
  • Author(s): 津田孝範
  • Organizer: 第14回日本抗加齢医学会総会シンポジウム
  • Place of Presentation: 大阪国際会議場（大阪府・大阪市）
  • Year and Date: 2014-06-06 – 2014-06-08
  • Invited
• [Presentation] クルクミンと消化管ホルモン分泌、糖尿病予防 (2014)
  • Author(s): 津田孝範
  • Organizer: 第68回日本栄養・食糧学会大会シンポジウム
  • Place of Presentation: 酪農学園大学（北海道・江別市）
  • Year and Date: 2014-05-30 – 2014-06-01
  • Invited
• [Presentation] GLP-1分泌を促進する食品因子とその機序解明 (2014)
  • Author(s): 加藤正樹、谷翼、津田孝範
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学（北海道・江別市）
  • Year and Date: 2014-05-30 – 2014-06-01
• [Book] Recent Advances in Polyphenol Research 5 (2017)
  • Author(s): Tsuda, T.
  • Total Pages: 376
  • Publisher: John Wiley & Sons, Inc.
• [Book] ポリフェノール：機能性成分研究開発の最新動向 (2016)
  • Author(s): Tsuda, T.
  • Total Pages: 289
  • Publisher: シーエムシー出版
• [Book] 食品因子による栄養制御 (2015)
  • Author(s): 加藤正樹、津田孝範
  • Total Pages: 283
  • Publisher: 建帛社
• [Book] 漬物の機能と科学 (2014)
  • Author(s): 津田孝範
  • Total Pages: 170
  • Publisher: 朝倉書店
• [Book] 食品学 (2014)
  • Author(s): 津田孝範
  • Total Pages: 166
  • Publisher: 朝倉書店