Effect of maternal excess fructose consumption on the offspring

• Project/Area Number: 26450169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Fujita Health University
• Principal Investigator: OHASI Koji 藤田保健衛生大学, 保健学研究科, 教授 (60278281)
• Co-Investigator(Kenkyū-buntansha): 宗綱 栄二 藤田保健衛生大学, 医学部, 助教 (30600431)
• Co-Investigator(Renkei-kenkyūsha): Yamada Hiroya 藤田保健衛生大学, 医学部, 助教 (80610352)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: フルクトース / エピジェネティクス / DNAメチル化 / 海馬 / 脱共役蛋白 / メチル化 / 次世代に対する影響 / 栄養

Outline of Final Research Achievements

Fructose is a dietary monosaccharide present naturally in fruits and vegetables. Fructose is often used as white crystalline table sugar in the form of refined sugars. The effect of maternal fructose on offspring was examined. Pregnant rats were fed with 20% fructose solution. Offspring delivered from fructose-fed dams showed impaired glucose tolerance after mature. Their weigh was lower than control offspring.
There are several evidences that brain function is sensitive to fructose consumption. Decreased Uncoupling protein 5 (UCP5) mRNA was observed in fructose offspring. The adverse effect of maternal fructose is mediated by the alternation of UCP5 mRNA expression. Offspring hippocampal function might be affected by maternal fructose. Now, we try to examine the adverse effect of fructose from the point of view of epigenetics.

Research Products

• [Journal Article] Fructose intake during gestation and lactation differentially affects the expression of hippocampal neurosteroidogenic enzymes in rat offspring (2017)
  • Author(s): Genki Mizuno, Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki1, Yuri Murase, Kanako Kondo, Hiroaki Ishikawa, Ryoji Teradaira, Koji Suzuki, Koji Ohashi
  • Journal Title: Endocrine Research Volume: 42 Issue: 1 Pages: 71-77
  • DOI: 10.1080/07435800.2016.1182186
  • Peer Reviewed / Open Access
• [Journal Article] Fructose intake during gestation and lactation differentially affects the expression of hippocampal neurosteroidogenic enzymes in rat offspring (2016)
  • Author(s): Genki Mizuno, Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Mirai Yamazaki1, Yuri Murase, Kanako Kondo, Hiroaki Ishikawa, Ryoji Teradaira, Koji Suzuki, Koji Ohashi,
  • Journal Title: Endocrine Research Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Maternal fructose consumption alters messenger RNA expression of hippocampal StAR, PBR, P450(11β), 11β-HSD, and 17β-HSD in rat offspring (2015)
  • Author(s): Koji Ohashi, Yoshitaka Ando, Eiji Munetsuna, Hiroya Yamada, Mirai Yamazaki, Ayuri Nagura, Nao Taromaru, Hiroaki Ishikawa, Koji Suzuki, Ryouji Teradaira
  • Journal Title: Nutrition research Volume: 35 Issue: 3 Pages: 259-264
  • DOI: 10.1016/j.nutres.2014.11.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 非アルコール性脂肪性肝疾患モデル動物におけるmiR-20a、miR27aの経時的変化 (2016)
  • Author(s): 山崎未来、山田宏哉、安藤嘉崇、近藤奏子、水野元貴、村瀬悠理、太田駿瑠、石川浩章、宗綱栄二、鈴木康司、大橋鉱二
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本（くまもと県民交流館パレア）
  • Year and Date: 2016-12-02
• [Presentation] 母獣のフルクトース過剰摂取が仔のコルチコステロン代謝に及ぼす影響 (2016)
  • Author(s): 村瀬悠理、宗綱栄二、山田宏哉、山崎未来、水野元貴、近藤奏子、太田駿瑠、石川浩章、寺平良治、鈴木康司、大橋鉱二
  • Organizer: 第56回日本臨床化学会年次学術集会
  • Place of Presentation: 熊本（くまもと県民交流館パレア）
  • Year and Date: 2016-12-02
• [Presentation] The establishment of simpler method for measuring HDL-miRNAs (2016)
  • Author(s): Kanako Kondo, Hiroaki Ishikawa, Hiroya Yamada, Mirai Yamazaki, Yuri Murase, Genki Mizuno, Takeru Ota, Eiji Munetsuna, Koji Suzuki, Ryoji Teradaira, Koji Ohashi
  • Organizer: 14th Asis-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress (APFCB 2016)
  • Place of Presentation: Taipei, TAIWAN（Taipei International Center)
  • Year and Date: 2016-11-26
  • Int'l Joint Research
• [Presentation] Maternal Fructose Consumption Alters Gene Expression Involved in Hepatic Glucocorticoid Metabolism in Rat Offspring (2016)
  • Author(s): Yuri Murase, Eiji Munetsuna, Hiroya Yamada, Mirai Yamazaki, Genki Mizuno, Kanako Kondo, Takeru Ota, Hiroaki Ishikawa, Ryoji Teradaira, Koji Suzuki, Koji Ohashi
  • Organizer: 14th Asis-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress (APFCB 2016)
  • Place of Presentation: Taipei, TAIWAN（Taipei International Center)
  • Year and Date: 2016-11-26
  • Int'l Joint Research
• [Presentation] 妊娠授乳期フルクトース過剰摂取モデルにおける仔の海馬酸化ストレス状態の解析 (2016)
  • Author(s): 水野元貴、村瀬悠理、山崎未来、近藤奏子、太田駿瑠、山田宏哉、宗綱栄二、石川浩章、寺平良治、鈴木康司、大橋鉱二
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2016-09-01
• [Presentation] 血中HDL-miRNA測定法の確立 (2016)
  • Author(s): 近藤奏子、石川浩章、山崎未来、村瀬悠理、水野元貴、太田駿瑠、山田宏哉、宗綱栄二、鈴木康司、寺平良治、大橋鉱二
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2016-09-01
• [Presentation] Basic Study on DNA Methylation Analysis - Targeting Promoter Region of Cytokine Genes in Human Whole Blood (2016)
  • Author(s): Genki Mizuno, Hiroya Yamada, Mirai Yamazaki, Takeru Ota, Yuri Murase, Kanako Kondo, Eiji Munetsuna, Hiroaki Ishikawa, Koji Ohashi, Koji Suzuki
  • Organizer: 14th Asis-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress (APFCB 2016)
  • Place of Presentation: Taipei, TAIWAN（Taipei International Center)
  • Int'l Joint Research
• [Presentation] High fructose consumption alters DNA methylation in rat liver. (2015)
  • Author(s): Koji Ohashi, Hiroya Yamada, Yoshitaka Ando, Eiji Munetsuna, Mitai Yamazaki, Genki Mizuno, Kanako Kondo, Hiroaki Ishikawa, Koji Suzuki, Ryoji Teradaira
  • Organizer: 21st IFCC-EFLM (European Congress of Clinical Chemistry and Laboratory Medicine) EUROLAB PARIS 2015
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Serum levels of miR-20a and miR-27a are associated with NAFLD in a general Japanese population: the Yakumo Study. (2015)
  • Author(s): Mitai Yamazaki, Koji Suzuki, Hiroya Yamada, Koji Ohashi, Naohiro Ichino, Hiroaki Ishikawa, Keisuke Osakabe, Keiko Sugimoto, Eiji Munetsuna, Genki Mizuno, Kanako Kondo, Takashi Inoue, Ryoji Teradaira
  • Organizer: 21st IFCC-EFLM (European Congress of Clinical Chemistry and Laboratory Medicine) EUROLAB PARIS 2015
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Stability of HDL-miRNA for pre-analytical conditions- (2015)
  • Author(s): Hiroaki Ishikawa, Nao Taromaru, Kanako Kondo, Mirai Yamazaki, Genki Mizuno, Hiroya Yamada, Koji Suzuki, Eiji Munetsuna, Koji Ohashi1, Ryoji Teradaira
  • Organizer: 21st IFCC-EFLM (European Congress of Clinical Chemistry and Laboratory Medicine) EUROLAB PARIS 2015
  • Place of Presentation: Paris, France
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Circulating microRNAs ,miR197, let7d, miR150, miR92, are influenced by smoking status in general population. (2014)
  • Author(s): Ayuri Nagura, Hiroya Yamada, Koji Ohashi, Hiroaki Ishikawa, Koji Suzuki, Naohiro Ichino, Yoshitaka Ando, Nao Taromaru, Mirai Yamazaki, Rika Watarai, Takashi Inoue, Nobuyuki Hamajima, Ryoji Teradaira
  • Organizer: 22nd International Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Ｉｓｔａｎｂｕｌｅ Ｃｏｎｇｒｅｓｓ Ｃｅｎｔｅｒ， Istanbul ,Turkey
  • Year and Date: 2014-06-22 – 2014-06-26
• [Presentation] Associations between circulating microRNAs miR-20a or miR-27a and non-alcoholic fatty liver disease in a general Japanese population: the Yakumo study. (2014)
  • Author(s): Mirai Yamazaki, Hiroya Yamada, Yoshitaka Ando, Koji Ohashi, Koji Suzuki, Naohiro Ichino, Hiroaki Ishikawa, Nao Taromaru, Ayuri Nagura, Keisuke Osakabe, Keiko Sugimoto, Takashi Inoue, Nobuyuki Hamajima, Ryoji Teradaira
  • Organizer: 22nd International Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Ｉｓｔａｎｂｕｌｅ Ｃｏｎｇｒｅｓｓ Ｃｅｎｔｅｒ，Istanbul ,Turkey
  • Year and Date: 2014-06-22 – 2014-06-26
• [Presentation] Analysis of the circulating HDL-miRNA stability for pre-analytical conditions (2014)
  • Author(s): Nao Taromaru, Hiroaki Ishikawa, Yoshitaka Ando, Ayuri Nagura, Mirai Yamazaki, Hiroya Yamada, Koji Suzuki, Koji Ohashi, Ryoji Teradaira
  • Organizer: 22nd International Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Ｉｓｔａｎｂｕｌｅ Ｃｏｎｇｒｅｓｓ Ｃｅｎｔｅｒ，Istanbul ,Turkey
  • Year and Date: 2014-06-22 – 2014-06-26