• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Control of pathogenic bacterial growth by using amino acid: Investigation of the working mechanism

Research Project

Project/Area Number 26450173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionHokkaido University

Principal Investigator

Koseki Shigenobu  北海道大学, 農学研究院, 准教授 (70414498)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsアミノ酸 / 適合溶質 / 非適合溶質 / 食塩 / 浸透圧ストレス / 予測モデル / 浸透圧 / 食塩ストレス / 食中毒細菌 / トリプトファン / Dトリプトファン / ストレス応答
Outline of Final Research Achievements

The present study firstly aimed at clarifying the effect of osmotic pressure induced by different osmolytes such as sodium chloride (NaCl), potassium chloride (KCl), and sucrose. Even at the same level of osmotic pressure, D-Tryptophan with NaCl significantly inhibited growth of Escherichia coli the most, compared with KCl and sucrose. Growth inhibition/no growth inhibition boundary conditions as a function of D-tryptophan and NaCl concentrations was clarified by a logistic regression model. The higher NaCl concentration, the lower D-tryptophan concentration was needed. Furthermore, we examined the effect of working temperature ranging from 15 to 46°C on the bacterial growth inhibition induced by D-tryptophan. The higher the reaction temperature, the more rapidly viable E. coli numbers were decreased. The results obtained in the present study would significantly contribute to the development of a novel bacterial growth control strategy using D-tryptophan.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 細菌のストレス応答機構の活用:アミノ酸添加による大腸菌の増殖抑制効果2016

    • Author(s)
      管快斗,川村周三,小関成樹
    • Organizer
      農業食料工学会2016年第75回年次大会
    • Place of Presentation
      京都大学(京都府,京都市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] D-Tryptophan acts as an incompatible solute: Effect of growth inhibition of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes2015

    • Author(s)
      Kaito Kan, Shige Koseki
    • Organizer
      29th EFFoST International Conference
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2015-11-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細菌のストレス応答機構の活用: アミノ酸添加による大腸菌の増殖抑制効果2015

    • Author(s)
      管快斗,長谷川真弓,川村周三,小関成樹
    • Organizer
      第74回農業食料工学会年次大会
    • Place of Presentation
      岩手大学 (岩手県,盛岡市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi