| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
This study was conducted to clarify that lactic acid bacteria which contain hydroxyl fatty acid have tolerance against stress conditions. Lactobacillus sakei Y-20 strain with ability to hydroxylate fatty acid and a standard strain of L. sakei were used for this study. First, their fatty acid hydroxylase was assayed. The Y-20 strain activity was found to be four times higher than that of standard L. sakei bacteria. Furthermore, the ratio of survival of Y-20 strain under stress caused by alcohol, pH, and metal ions was found to be higher. After the strain-related fatty acid hydroxylase gene was prepared, its effects were investigated. Results show that the hydroxylase decreased tolerance against stress, and that the respective expressions of cell membrane transporter proteins and metabolisms of fatty acid were decreased also. We infer from the results that the fatty acid hydroxyl supports stress tolerance.
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