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Studies on roles and formation mechanisms of taste-active hydrophobic compounds in sake.

Research Project

Project/Area Number 26450181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionAkita Prefectural University

Principal Investigator

Hashizume Katsumi  秋田県立大学, 生物資源科学部, 教授 (30372189)

Co-Investigator(Kenkyū-buntansha) 伊藤 俊彦  秋田県立大学, 生物資源科学部, 助教 (50336442)
Co-Investigator(Renkei-kenkyūsha) Okuda Masaki  独立行政法人酒類総合研究所, 主任研究員 (80372192)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords清酒 / 疎水性呈味成分 / フェルラ酸 / フェルラ酸エチル / エチルエステル化ペプチド / ピログルタミルデカペプチドエチルエステル
Outline of Final Research Achievements

Forming mechanisms of ferulic acid (FA) and ethyl ferulate (FE) under sake mash conditions were studied, and affecting factors of these compound levels in the sake were elucidated. It was also found that feruloylated oligosaccharide forming enzyme(s) from Aspergillus oryzae may play a crucial role in the forming levels of FE and FA (especially FE/FA ratio) in the sake brewing process. A new accurate quantitation method for taste-active pyroglutamyl decapeptide ethyl esters (PGDPEs) in sake was developed by using a SIDA method, and then thresholds of PGDPEs in the sake were estimated. The results revealed that PGDPEs may play significant sensory roles in the sake taste.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Quantitation using a stable isotope dilution assay (SIDA) and thresholds of taste-active pyroglutamyl decapeptide ethyl esters (PGDPEs) in sake2017

    • Author(s)
      Katsumi Hashizume, Toshiko Ito and Shinya Igarashi
    • Journal Title

      Bioscience and Biotchnology and Biochemistry

      Volume: 81 Issue: 3 Pages: 426-430

    • DOI

      10.1080/09168451.2016.1259554

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Formation of guaiacol by spoilage bacteria from vanillic acid, a pdoduct of rice koji cultivation, in Japanese sake brewing2016

    • Author(s)
      Toshihiko Ito, Mahito Konno, Yoichro Shimura, Seiei Watanabe, Hitoshi Takahashi, Katsumi Hashizume
    • Journal Title

      Jouranal of Agricultural and Food Chemistry

      Volume: 64 Issue: 22 Pages: 4599-4605

    • DOI

      10.1021/acs.jafc.6b01031

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fromation of ethyl ferulate from feruloylated oligosaccharide by transesterification of rice koji enzyme under sake mash conditions2016

    • Author(s)
      Nobukazu Suzuki, Toshihiko Ito, Kai Hiroshima, Tetsuo Tokiwano, Katsumi Hashizume
    • Journal Title

      Jounal of Bioscience and Bioengineering

      Volume: 121 Issue: 3 Pages: 281-285

    • DOI

      10.1016/j.jbiosc.2015.06.015

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Volatile Phenolic Compounds Responsible for 4-vinylguaiacol-like Odor Characteristics of Sake2016

    • Author(s)
      Miyuki Sunao, Toshihiko Ito, Sato Masa, Tomomi Uehara, Tsuyoshi Ohno, Seiei Watanabe, Hitoshi Takahashi, Katsumi Hashizume
    • Journal Title

      Food Science and Technology Research

      Volume: 22 Issue: 1 Pages: 111-116

    • DOI

      10.3136/fstr.22.111

    • NAID

      130005131919

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] 清酒と原料米のフェノール酸関連化合物2015

    • Author(s)
      橋爪克己
    • Journal Title

      日本醸造協会誌

      Volume: 110 Pages: 2-7

    • NAID

      130006694382

    • Related Report
      2014 Research-status Report
  • [Journal Article] Factors affecting phenolic acid liberation from rice grains in the sake brewing process2014

    • Author(s)
      Toshihiko Ito, Nobukazu Suzuki, Airi Nakayama, Masaya Ito, Katsumi Hashizume
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 118 Pages: 640-645

    • NAID

      110009900847

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 清酒中の呈味性ピログルタミルデカペプチドエチルエステル(PGDPEs)の安定同位体希釈分析(SIDA)法による定量と閾値2017

    • Author(s)
      橋爪克己、五十嵐信也、高橋樹、伊藤俊彦
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 清酒中の苦味成分に関する研究(日本醸造協会技術賞受賞講演)2016

    • Author(s)
      橋爪克己、伊藤俊彦
    • Organizer
      平成28年度日本醸造学会大会
    • Place of Presentation
      北とぴあ(東京都北区)
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 清酒醸造におけるグアイアコール(4-VG様フェノール臭原因物質)の生成2016

    • Author(s)
      今野真人、伊藤俊彦、志村洋一郎、渡邉誠衛、高橋仁、橋爪克己
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 清酒の4-ビニルグアイアコール様臭の原因となる揮発性フェノール化合物の解析2015

    • Author(s)
      直みゆき、伊藤俊彦、廣嶋魁、佐藤雅、上原智美、大野剛、渡邉誠衛、高橋仁、橋爪克己
    • Organizer
      平成27年度日本醸造学会
    • Place of Presentation
      北とぴあ
    • Year and Date
      2015-10-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 清酒のフェルラ酸およびフェルラ酸エチルレベルの変動要因について2015

    • Author(s)
      鈴木伸和、廣嶋魁、高野康人、伊藤俊彦、橋爪克己
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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