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Elucidation of SS bond formation mechanism in bread making

Research Project

Project/Area Number 26450182
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionTokyo University of Agriculture

Principal Investigator

NOGUCHI TOMOHIRO  東京農業大学, 応用生物科学部, 教授 (80297598)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords製パン / PDI / ジスルフィド結合 / パン / アスコルビン酸 / ERO1 / SS結合
Outline of Final Research Achievements

The PDI activity was different between super strong flour, strong flour and medium flour, and strong flour showed high activity. In addition, the PDI activity in the wheat flour reached a peak at 20 to 25 days after anthesis at the late stage of wheat grain ripening and then showed a tendency to decrease. It was inferred that the PDI expressed at this time remained in the wheat flour and was involved in S - S bond formation in the dough. Analysis of the S - S bond formation state in the wheat dough suggested that intermolecular S - S bonds were formed in the 30 - 40 kDa protein and the molecular weight was increased.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] Influence of disulfide bond formation via PDI-Ero1 processing of proteins and baking quality2015

    • Author(s)
      NOGUCHI Tomohiro, NISNIBORI Fumiya, SHIONO Koji, OKA Daiki, NOGUCHI Haruko, TAKANO Katsumi
    • Journal Title

      Food Preservation Science

      Volume: 41 Pages: 247-252

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 小麦粉生地中におけるSS結合形成に対する酸化剤とPDI及びPODの影響について2016

    • Author(s)
      樋口 貴俊,岡 大貴,野口 治子,野口 智弘,高野 克己
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 小麦登熟過程中のPDI活性の挙動解析2016

    • Author(s)
      加藤 亮、野口 智弘、高野 克己
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 小麦粉中のプロテインジスルフィドイソメラーゼ活性の低下と小麦粉の製パン性の関係について2015

    • Author(s)
      樋口貴俊,岡 大貴,野口治子,野口智弘,高野克己
    • Organizer
      日本食品科学工学会第62回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-08-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 小麦の栽培条件の差異によるプロテインジスルフィドイソメラーゼ活性量の比較解析2015

    • Author(s)
      加藤 亮
    • Organizer
      日本食品保蔵学会創立40周年記念大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-06-27 – 2015-06-28
    • Related Report
      2014 Research-status Report
  • [Presentation] コムギ「 さとのそら 」の登熟過程におけるPDI ファ ミリーの発現量解析2014

    • Author(s)
      徳本 脩
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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