| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
To verify reproductive interference in Chamaecyparis pisifera by heterospecific pollens from artificial stands of Cryptomeria japonica and Ch. obutusa, reproductive isolation among the three Cupressaceae species and species composition of airborne pollens were investigated.
The results from the withdrawal of pollination drop and post pollination behavior of the heterospecific pollen tubes in the ovules suggest that reproductive isolation among the three species did not function at the pollination stage but in the post-pollination stage.
To evaluate species composition of airborne pollen, chloroplast DNA based genetic markers and single-pollen genotyping method was developed. Cupressaceae pollens were isolated by microscope and genotyped with the markers. The 90% of the Cupressaceae pollen was identified as Ch. obutusa. These results suggest that Ch. pisifera might suffer reproductive interference due to the heavier contamination of heterospecific pollens from related species.
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