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Genome and genetic analysis of the interactions between host (Pine tree), pathogen (pine wood nematode), and bacteria: focused on the oxidative stress responses

Research Project

Project/Area Number 26450204
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionChubu University

Principal Investigator

HASEGAWA Koichi  中部大学, 応用生物学部, 准教授 (10609837)

Co-Investigator(Kenkyū-buntansha) 河合 都妙  中部大学, 応用生物学部, 助教 (90610717)
Research Collaborator IKUYO Yoriko  中部大学, 応用生物学部, 助手
TAYAMACHI Akihiro  中部大学, 応用生物学研究科, 大学院生
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsマツ枯れ病 / マイクロバイオーム / ゲノム / 酸化バースト / 過酸化水素水 / 遺伝子ノックアウト / マツノザイセンチュウ / 植物内生細菌 / 細菌
Outline of Final Research Achievements

The pine wilt disease continues devastative influence on the forest ecosystem of the world. The causal agent is the pine wood nematode Bursaphelenchus xylophilus, spread mainly in East Asia and Europa by Monochamus beetles. In this project, we showed the dynamics of microbiomes under the pine wilt disease environment. We also revealed its unique ecological function by molecular genetic approaches, focusing on the oxidative stress response that is the initial defense reaction in plants against pathogenic infection.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2016 2015 2014 Other

All Int'l Joint Research (5 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 6 results) Presentation (8 results) (of which Int'l Joint Research: 3 results) Remarks (3 results)

  • [Int'l Joint Research] University of Evora(Portugal)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Academia Sinica(Taiwan)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] The James Hutton Institute(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Evora(Portugal)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] The James Hutton Institute(United Kingdom)

    • Related Report
      2015 Research-status Report
  • [Journal Article] The Genome and Genetics of a High Oxidative Stress Tolerant Serratia sp. LCN16 Isolated From the Plant Parasitic Nematode Bursaphelenchus xylophilus2016

    • Author(s)
      Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Cock, P.J.A., Mota, M., Hasegawa, K.
    • Journal Title

      BMC Genomics

      Volume: 17 Issue: 1 Pages: 301-301

    • DOI

      10.1186/s12864-016-2626-1

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evidences for an opportunistic and endophytic lifestyle of the Bursaphelenchus xylophilus-associated bacteria Serratia marcescens PWN146 isolated from wilting Pinus pinaster2016

    • Author(s)
      Vicente, C.S.L., Nascimento, F.X., Barbosa, P., Huei-Mien, K., Tsai, I.J., Hirao, T., Cock, P.J.A., Kikuchi, T., Hasegawa, K., Mota, M.
    • Journal Title

      Microbial Ecology

      Volume: 72 Issue: 3 Pages: 669-681

    • DOI

      10.1007/s00248-016-0820-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bacterial community associated to the pine wilt disease insect vectors Monochamus galloprovincialis and Monochamus alternatus2016

    • Author(s)
      Alves, M., Pereira, A., Matos, P., Henriques, J., Vicente, C., Aikawa, T., Hasegawa, K., Nascimento, F., Mota, M., Correia, A., Henriques, I.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 23908-23908

    • DOI

      10.1038/srep23908

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Catalases induction in high virulence pinewood nematode Bursaphelenchus xylophilus under hydrogen peroxide-induced stress2015

    • Author(s)
      Vicente CSL, Ikuyo Y, Shinya R, Mota M, Hasegawa K
    • Journal Title

      PLoS ONE

      Volume: なし Issue: 4 Pages: e0123839-e0123839

    • DOI

      10.1371/journal.pone.0123839

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bacterial role in pine wilt disease development -review and future perspectives2015

    • Author(s)
      Nascimento, F.X., Hasegawa, K., Mota, M., Vicente, C.S.L.
    • Journal Title

      Environmental Microbiology Reports

      Volume: 7 Issue: 1 Pages: 51-63

    • DOI

      10.1111/1758-2229.12202

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Direct interaction between the WD40 repeat protein WDR-23 and SKN-1/Nrf inhibits binding to target DNA2014

    • Author(s)
      Leung, C.K., Hasegawa, K., Wang, Y., Deonarine, A., Tang, L., Miwa, J., Choe, K.P.
    • Journal Title

      Molecular and Cellular Biology

      Volume: 34 Issue: 16 Pages: 3156-3167

    • DOI

      10.1128/mcb.00114-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] New insights into the biology of PWN-associated bacteria: genome analysis of Serratia sp. LCN16 and Serratia marcescens PWN1462016

    • Author(s)
      Vicente, C.S.L., Mota, M., Hasegawa, K.
    • Organizer
      IUFRO Pine Wilt Disease Symposium 2016
    • Place of Presentation
      Seoul (South Korea)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Genome and Genetics of a High Oxidative Stress Tolerant Serratia sp. LCN16 isolated from the plant parasitic nematode Bursaphelenchus xylophilus2016

    • Author(s)
      Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Mota, M., Hasegawa, K.
    • Organizer
      ASM Microbe 2016
    • Place of Presentation
      Boston, MA (USA)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genomic analysis of nematode-bacteria interaction in Pine Wilt Disease2015

    • Author(s)
      Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Peter, C., Mota, M., Hasegawa, K.
    • Organizer
      日本線虫学会第23回大会
    • Place of Presentation
      中部大学三浦記念会館(愛知県)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Research-status Report
  • [Presentation] Study of nematode-bacteria interaction in Pine Wilt Disease2015

    • Author(s)
      Vicente, C.S.L., Nascimento F., Ikuyo, Y., Mota, M., Hasegawa, K.
    • Organizer
      6th Congress of European Microbiologists
    • Place of Presentation
      Maastricht (The Netherlands)
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゲノムから見るマツ枯れ病関連細菌Serratia marcescens PWN-146の役割2015

    • Author(s)
      Vicente,C., 田山地晃宏, Cock, P., 幾代以子, Mota, M., 長谷川浩一
    • Organizer
      第59回日本応用動物昆虫学会大会
    • Place of Presentation
      山形大学、山形市、山形県
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Lack of the function for oxidative stress response in the pine wood nematode Bursaphelenchus xylophilus low virulent strain2014

    • Author(s)
      Vicente, C.S.L., Soga, K., Shinya, R., Mota, M., Hasegawa, K.
    • Organizer
      日本線虫学会第22回大会
    • Place of Presentation
      文部科学省研究交流センター、つくば市、茨城県
    • Year and Date
      2014-09-16 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Virulence and oxidative stress response of the pine wood nematode Bursaphelenchus xylophilus2014

    • Author(s)
      Vicente, C.S.L., Ikuyo, Y., Shinya, R., Mota, M., Hasegawa, K.
    • Organizer
      6th International Congress of Nematology
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2014-05-04 – 2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Ecological role of bacteria associated with the pine wilt disease system2014

    • Author(s)
      Francisco X. Nascimento, Cláudia S.L. Vicente, Pedro Barbosa, Margarida Espada, Paulo Vieira, Koichi Hasegawa, Manuel Mota
    • Organizer
      6th International Congress of Nematology
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2014-05-04 – 2014-05-09
    • Related Report
      2014 Research-status Report
  • [Remarks] 中部大学応用生物学長谷川浩一研究室

    • URL

      http://www3.chubu.ac.jp/faculty/hasegawa_koichi/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 中部大学応用生物学部長谷川浩一研究室

    • URL

      http://www3.chubu.ac.jp/faculty/hasegawa_koichi/

    • Related Report
      2015 Research-status Report
  • [Remarks] 中部大学長谷川浩一研究室

    • URL

      http://www3.chubu.ac.jp/faculty/hasegawa_koichi/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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