Genome and genetic analysis of the interactions between host (Pine tree), pathogen (pine wood nematode), and bacteria: focused on the oxidative stress responses

• Project/Area Number: 26450204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Chubu University
• Principal Investigator: HASEGAWA Koichi 中部大学, 応用生物学部, 准教授 (10609837)
• Co-Investigator(Kenkyū-buntansha): 河合 都妙 中部大学, 応用生物学部, 助教 (90610717)
• Research Collaborator: IKUYO Yoriko 中部大学, 応用生物学部, 助手 ; TAYAMACHI Akihiro 中部大学, 応用生物学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: マツ枯れ病 / マイクロバイオーム / ゲノム / 酸化バースト / 過酸化水素水 / 遺伝子ノックアウト / マツノザイセンチュウ / 植物内生細菌 / 細菌

Outline of Final Research Achievements

The pine wilt disease continues devastative influence on the forest ecosystem of the world. The causal agent is the pine wood nematode Bursaphelenchus xylophilus, spread mainly in East Asia and Europa by Monochamus beetles. In this project, we showed the dynamics of microbiomes under the pine wilt disease environment. We also revealed its unique ecological function by molecular genetic approaches, focusing on the oxidative stress response that is the initial defense reaction in plants against pathogenic infection.

Research Products

• [Int'l Joint Research] University of Evora(Portugal)
• [Int'l Joint Research] Academia Sinica(Taiwan)
• [Int'l Joint Research] The James Hutton Institute(英国)
• [Int'l Joint Research] University of Evora(Portugal)
• [Int'l Joint Research] The James Hutton Institute(United Kingdom)
• [Journal Article] The Genome and Genetics of a High Oxidative Stress Tolerant Serratia sp. LCN16 Isolated From the Plant Parasitic Nematode Bursaphelenchus xylophilus (2016)
  • Author(s): Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Cock, P.J.A., Mota, M., Hasegawa, K.
  • Journal Title: BMC Genomics Volume: 17 Issue: 1 Pages: 301-301
  • DOI: 10.1186/s12864-016-2626-1
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evidences for an opportunistic and endophytic lifestyle of the Bursaphelenchus xylophilus-associated bacteria Serratia marcescens PWN146 isolated from wilting Pinus pinaster (2016)
  • Author(s): Vicente, C.S.L., Nascimento, F.X., Barbosa, P., Huei-Mien, K., Tsai, I.J., Hirao, T., Cock, P.J.A., Kikuchi, T., Hasegawa, K., Mota, M.
  • Journal Title: Microbial Ecology Volume: 72 Issue: 3 Pages: 669-681
  • DOI: 10.1007/s00248-016-0820-y
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bacterial community associated to the pine wilt disease insect vectors Monochamus galloprovincialis and Monochamus alternatus (2016)
  • Author(s): Alves, M., Pereira, A., Matos, P., Henriques, J., Vicente, C., Aikawa, T., Hasegawa, K., Nascimento, F., Mota, M., Correia, A., Henriques, I.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 23908-23908
  • DOI: 10.1038/srep23908
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Catalases induction in high virulence pinewood nematode Bursaphelenchus xylophilus under hydrogen peroxide-induced stress (2015)
  • Author(s): Vicente CSL, Ikuyo Y, Shinya R, Mota M, Hasegawa K
  • Journal Title: PLoS ONE Volume: なし Issue: 4 Pages: e0123839-e0123839
  • DOI: 10.1371/journal.pone.0123839
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bacterial role in pine wilt disease development -review and future perspectives (2015)
  • Author(s): Nascimento, F.X., Hasegawa, K., Mota, M., Vicente, C.S.L.
  • Journal Title: Environmental Microbiology Reports Volume: 7 Issue: 1 Pages: 51-63
  • DOI: 10.1111/1758-2229.12202
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Direct interaction between the WD40 repeat protein WDR-23 and SKN-1/Nrf inhibits binding to target DNA (2014)
  • Author(s): Leung, C.K., Hasegawa, K., Wang, Y., Deonarine, A., Tang, L., Miwa, J., Choe, K.P.
  • Journal Title: Molecular and Cellular Biology Volume: 34 Issue: 16 Pages: 3156-3167
  • DOI: 10.1128/mcb.00114-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] New insights into the biology of PWN-associated bacteria: genome analysis of Serratia sp. LCN16 and Serratia marcescens PWN146 (2016)
  • Author(s): Vicente, C.S.L., Mota, M., Hasegawa, K.
  • Organizer: IUFRO Pine Wilt Disease Symposium 2016
  • Place of Presentation: Seoul (South Korea)
  • Year and Date: 2016-08-29
  • Int'l Joint Research
• [Presentation] The Genome and Genetics of a High Oxidative Stress Tolerant Serratia sp. LCN16 isolated from the plant parasitic nematode Bursaphelenchus xylophilus (2016)
  • Author(s): Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Mota, M., Hasegawa, K.
  • Organizer: ASM Microbe 2016
  • Place of Presentation: Boston, MA (USA)
  • Year and Date: 2016-06-16
  • Int'l Joint Research
• [Presentation] Genomic analysis of nematode-bacteria interaction in Pine Wilt Disease (2015)
  • Author(s): Vicente, C.S.L., Nascimento, F.X., Ikuyo, Y., Peter, C., Mota, M., Hasegawa, K.
  • Organizer: 日本線虫学会第23回大会
  • Place of Presentation: 中部大学三浦記念会館（愛知県）
  • Year and Date: 2015-09-02
• [Presentation] Study of nematode-bacteria interaction in Pine Wilt Disease (2015)
  • Author(s): Vicente, C.S.L., Nascimento F., Ikuyo, Y., Mota, M., Hasegawa, K.
  • Organizer: 6th Congress of European Microbiologists
  • Place of Presentation: Maastricht （The Netherlands）
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] ゲノムから見るマツ枯れ病関連細菌Serratia marcescens PWN-146の役割 (2015)
  • Author(s): Vicente，C., 田山地晃宏, Cock, P., 幾代以子, Mota, M., 長谷川浩一
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学、山形市、山形県
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Lack of the function for oxidative stress response in the pine wood nematode Bursaphelenchus xylophilus low virulent strain (2014)
  • Author(s): Vicente, C.S.L., Soga, K., Shinya, R., Mota, M., Hasegawa, K.
  • Organizer: 日本線虫学会第22回大会
  • Place of Presentation: 文部科学省研究交流センター、つくば市、茨城県
  • Year and Date: 2014-09-16 – 2014-09-18
• [Presentation] Virulence and oxidative stress response of the pine wood nematode Bursaphelenchus xylophilus (2014)
  • Author(s): Vicente, C.S.L., Ikuyo, Y., Shinya, R., Mota, M., Hasegawa, K.
  • Organizer: 6th International Congress of Nematology
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-05-04 – 2014-05-09
• [Presentation] Ecological role of bacteria associated with the pine wilt disease system (2014)
  • Author(s): Francisco X. Nascimento, Cláudia S.L. Vicente, Pedro Barbosa, Margarida Espada, Paulo Vieira, Koichi Hasegawa, Manuel Mota
  • Organizer: 6th International Congress of Nematology
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-05-04 – 2014-05-09
• [Remarks] 中部大学応用生物学長谷川浩一研究室
  • URL: http://www3.chubu.ac.jp/faculty/hasegawa_koichi/
• [Remarks] 中部大学応用生物学部長谷川浩一研究室
  • URL: http://www3.chubu.ac.jp/faculty/hasegawa_koichi/
• [Remarks] 中部大学長谷川浩一研究室
  • URL: http://www3.chubu.ac.jp/faculty/hasegawa_koichi/