| Project/Area Number |
26450234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tokyo Kasei University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小松 あき子 東京家政大学, 家政学部, 助教 (20649371)
近藤 秀樹 岡山大学, 資源植物科学研究所, 准教授 (40263628)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ウイルス / 担子菌 / 菌類ウイルス / 遺伝子発現 / マイクロアレイ / 子実体 / シグナル / キノコ / マイタケ / G frondosa / RNA virus / 発現解析 / 薬剤耐性 / ウイルスフリー / non capcid virus / ゲノム / シクロヘキシミド / Grifola |
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| Outline of Final Research Achievements |
We isolated two mycoviruses from Grifola flondosa (MAITAKE), and finally identified two viruses as Parititivirus (GfPV1) and RNA Virus (GfRV1). GfPV1 has a capcid protein gene and RdRp protein. And we successfully to purified virions with high centrifugation method and with its sequences, finally we identified as new type of Parititivirus in Grifola flondosa. On the other hand, GfRV1 possibly has no coat proteins, thus tentatively we identified as capcidless virus. Both of them behave as no phenotypic effects as host of the G. flondosa, but treated with cell cycle arrest chemical and low temperature harvest influence its growth. And we created virus free hosts and re-infectious each viruses respectively hosts, thus we are now start to analysis of virus positive vs. virus free hosts gene expression analysis as a microarray. This results will be future study.
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