| Project/Area Number |
26450237
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | Kindai University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUKUTA Yasuhisa 近畿大学, 農学部, 講師 (80609602)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | グルタミン酸脱炭酸酵素 / フォスファチジルセリン脱炭酸酵素 / GABA / シイタケ / マイタケ / エノキタケ / クルタミン酸脱炭酸酵素 |
|---|
| Outline of Final Research Achievements |
In order to evaluate the mechanisms for gamma-aminobutyric acid (GABA) accumulation in edible mushroom, we purified and characterized the enzymes for GABA formation from Grifola frondosa, Lentinula edodes and Flammulina velutipes. The enzymes from G. frondosa, and F. velutipes enzyme used only L-glutamic acid as substrate for decarboxylation reaction so that these enzymes should belong to glutamic acid decarboxylase. However, the enzyme from L. edodes can use aspartic acid, other than glutamic acid, as substrate for the reaction. The internal amino acid sequences of this enzyme showed that the enzyme should be phosphatidylserine decarboxylase (PSD) of L. edodes. However, the reaction of PSD couldn’t be detected.
|
