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Is organic matter degradation in aquatic ecosystem attributed only to heterotrophic bacteria?

Research Project

Project/Area Number 26450245
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionEhime University (2015-2016)
University of Tsukuba (2014)

Principal Investigator

OBAYASHI Yumiko  愛媛大学, 沿岸環境科学研究センター, 助教 (60380284)

Co-Investigator(Renkei-kenkyūsha) TAKAO Yoshitake  福井県立大学, 海洋生物資源学部, 准教授 (00511304)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords海洋生態系 / 有機物分子変換 / 海洋微生物群集 / 従属栄養性細菌 / 原生生物 / 細胞外加水分解酵素 / 微生物ループ / 物質循環 / 細胞外酵素
Outline of Final Research Achievements

Among biogeochemical processes in aquatic environment, degradation and/or transformation of natural organic matter in aquatic ecosystem is one of the most undescribed processes so far. Degradation of organic matter in aquatic ecosystem is generally thought to be attributed to heterotrophic bacteria, however, we hypothesized that not only heterotrophic bacteria but also eukaryotic microbes, especially protists, contribute to organic matter degradation processes in aquatic environment. In this study, we found that some kinds of protists showed high potential activities of extracellular proteases and parts of their enzymes were seemed to be secreted to the surrounding water. Further researches for the contribution of the protists to organic matter degradation in aquatic environment are expected.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Extracellular proteases are released by ciliates in defined seawater microcosms2015

    • Author(s)
      Thao NV, Nozawa A, Obayashi Y, Kitamura S-I, Yokokawa T, Suzuki S
    • Journal Title

      Marine Environmental Research

      Volume: 109 Pages: 95-102

    • DOI

      10.1016/j.marenvres.2015.06.011

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Coexisting protist-bacterial community accelerates protein transformation in microcosm experiments2014

    • Author(s)
      Thao NV, Obayashi Y, Yokokawa T, Suzuki S
    • Journal Title

      Frontiers in Marine Science

      Volume: 1:69 Pages: 1-9

    • DOI

      10.3389/fmars.2014.00069

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] クラゲ遺骸の微生物分解に関する研究2016

    • Author(s)
      今中加奈,大林由美子,宗林留美,西川 淳
    • Organizer
      富士山麓アカデミック&サイエンスフェア2016
    • Place of Presentation
      ふじさんめっせ(静岡県富士市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 真核微生物ラビリンチュラ類の細胞外プロテアーゼプロファイル ~水圏での有機物分解者としてのポテンシャルを探るために~2016

    • Author(s)
      大林由美子,高尾祥丈
    • Organizer
      日本微生物生態学会 第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Proteolytic enzymes in seawater: contribution of prokaryotes and protists2016

    • Author(s)
      Yumiko Obayashi, Satoru Suzuki
    • Organizer
      2016 Ocean Sciences Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2016-02-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ラビリンチュラ類のタンパク質分解酵素活性2015

    • Author(s)
      高尾祥丈,松岡順平,大林由美子
    • Organizer
      ラビリンチュラ・シンポジウム
    • Place of Presentation
      日本科学未来館(東京都江東区)
    • Year and Date
      2015-07-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 細菌だけではないみたい ―海水中の有機物分解酵素の源―2015

    • Author(s)
      大林 由美子, Ngo Vy Thao, 北村 真一, 横川 太一, 鈴木 聡
    • Organizer
      日本海洋学会2015年度春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス(東京都港区)
    • Year and Date
      2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Bacterial proteins are rapidly processed in the presence of both bacteria and ciliates2014

    • Author(s)
      Thao N.V., A. Nozawa, Y. Obayashi, S-I. Kitamura, T. Yokokawa, S. Suzuki
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 生物活性阻害剤を用いて海水中のタンパク質分解酵素活性の起源を探る2014

    • Author(s)
      大林 由美子, 横川 太一, 鈴木 聡
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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