Developmental study of marine virus monitoring method in natural sea area.

• Project/Area Number: 26450249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: The Nippon Dental University (2015-2016); Keio University (2014)
• Principal Investigator: Toyoda Kensuke 日本歯科大学, 生命歯学部, 非常勤講師 (40585874)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 海洋ウイルス / 珪藻 / リアルタイムqPCR / ELISA / 定量検出 / ELISA法 / Real-time qPCR / 藻類 / リアルタイムRT-PCR法 / marine virus / diatom virus / phycology / Real-time RT-qPCR / Diatom / Marine Virus / Phycology / Virology

Outline of Final Research Achievements

A monitoring method in a more sensitive and stable natural sea area for three new viruses infected with marine floating diatoms, Cylindrotheca closterium, have developed. During the experiment, host real-time qPCR, genome analysis of three new viruses, development of real-time qRT-PCR, and development of a virus quantitative detection method by ELISA were performed.
As a result, method of stable and good quantification / detection sensitivity from natural sea water by real-time qRT-PR method and Direct ELISA method have developed.